LOCUS       sequence-117104-         997 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  GFPuv Expression, Low Phosphate Dependent E. coli Expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 997)
  AUTHORS   Menacho-Melgar R, Ye Z, Moreb EA, Yang T, Efromson JP, Decker JS, 
            Wang R, Lynch MD
  TITLE     Scalable, two-stage, autoinduction of recombinant protein expression
            in E. coli utilizing phosphate depletion.
  JOURNAL   Biotechnol Bioeng. 2020 May 22. doi: 10.1002/bit.27440.
  PUBMED    32441815
REFERENCE   2  (bases 1 to 997)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2020 May 22. doi: 10.1002/bit.27440."
COMMENT     Sequence Label: pSMART-GFPuv
FEATURES             Location/Qualifiers
     source          1..997
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(33..62)
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
     terminator      146..217
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      233..260
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     primer_bind     complement(575..603)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 aaggatctca agaagatcct ttgattttct accgaagaaa ggcccacccg tgaaggtgag
       61 ccagtgagtt gattgcagtc cagttacgct ggagtctgag gctcgtcctg aatgatatca
      121 agcttgaatt cgtttgccca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc
      181 ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc
      241 ttcgggtggg cctttctgcg tttatacaca gctaacacca cgtcgtccct atctgctgcc
      301 ctaggtctat gagtggttgc tggataacgt gcgtaattgt gctgatctct tatatagctg
      361 ctctcattat ctctctaccc tgaagtgact ctctcacctg taaaaataat atctcacagg
      421 cttaatagtt tcttaataca aagcctgtaa aacgtcagga taacttctat attcagggag
      481 accacaacgg tttccctcta caaataattt tgtttaactt tcgtgtgtag gaggataatc
      541 tatggctagc aaaggagaag aacttttcac tggagttgtc ccaattcttg ttgaattaga
      601 tggtgatgtt aatgggcaca aattttctgt cagtggagag ggtgaaggtg atgctacata
      661 cggaaagctt acccttaaat ttatttgcac tactggaaaa ctacctgttc catggccaac
      721 acttgtcact actttctctt atggtgttca atgcttttcc cgttatccgg atcatatgaa
      781 acggcatgac tttttcaaga gtgccatgcc cgaaggttat gtacaggaac gcactatatc
      841 tttcaaagat gacgggaact acaagacgcg tgctgaagtc aagtttgaag gtgataccct
      901 tgttaatcgt atcgagttaa aaggtattga ttttaaagaa gatggaaaca ttctcggaca
      961 caaactcgag tacaactata actcacacaa tgtatac
//
