LOCUS       sequence-117109-         733 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of C terminally GFP-tagged C11orf83/UQCC3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 733)
  AUTHORS   Desmurs M, Foti M, Raemy E, Vaz FM, Martinou JC, Bairoch A, Lane L
  TITLE     C11orf83, a mitochondrial cardiolipin-binding protein involved in 
            bc1 complex assembly and supercomplex stabilization.
  JOURNAL   Mol Cell Biol. 2015 Apr;35(7):1139-56. doi: 10.1128/MCB.01047-14. 
            Epub 2015 Jan 20.
  PUBMED    25605331
REFERENCE   2  (bases 1 to 733)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/MCB.01047-14"; journalName: "Mol Cell Biol"; date: 
            "2015-04"; volume: "35"; issue: "7"; pages: "1139-56"
COMMENT     Sequence Label: pcDNA-Dest47- C11orf83-GFP
FEATURES             Location/Qualifiers
     source          1..733
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     50..69
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        50..68
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    101..125
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     RBS             137..157
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    complement(462..486)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(530..558)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
       61 actataggga gacccaagct ggctagttaa gcttgatcaa acaagtttgt acaaaaaagc
      121 aggctccgcg gccgccttgt ttaactttaa gaaggagccc ttcaccatgg attccttgcg
      181 gaaaatgctg atctcagtcg caatgctggg cgcaggggct ggcgtgggct acgcgctcct
      241 cgttatcgtg accccgggag agcggcggaa gcaggaaatg ctaaaggaga tgccactgca
      301 ggacccaagg agcagggagg aggcggccag gacccagcag ctattgctgg ccactctgca
      361 ggaggcagcg accacgcagg agaacgtggc ctggaggaag aactggatgg ttggcggcga
      421 aggcggcgcc ggcgggaggt caccgaaggg tgggcgcgcc gacccagctt tcttgtacaa
      481 agtggttcga tctagaatgg ctagcaaagg agaagaactt ttcactggag ttgtcccaat
      541 tcttgttgaa ttagatggtg atgttaatgg gcacaaattt tctgtcagtg gagagggtga
      601 aggtgatgct acatacggaa agcttaccct taaatttatt tgcactactg gaaaactacc
      661 tgttccatgg ccaacacttg tcactacttt ctcttatggt gttcaatgct tttcccgtta
      721 tccggatcat atg
//
