LOCUS       sequence-117110-         757 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of the mutated N terminal part (R5D-K5D N23, 
            transmembrane part of 23 aa) of C11orf83/UQCC3 fused to GFP protein 
            (C-ter).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 757)
  AUTHORS   Desmurs M, Foti M, Raemy E, Vaz FM, Martinou JC, Bairoch A, Lane L
  TITLE     C11orf83, a mitochondrial cardiolipin-binding protein involved in 
            bc1 complex assembly and supercomplex stabilization.
  JOURNAL   Mol Cell Biol. 2015 Apr;35(7):1139-56. doi: 10.1128/MCB.01047-14. 
            Epub 2015 Jan 20.
  PUBMED    25605331
REFERENCE   2  (bases 1 to 757)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/MCB.01047-14"; journalName: "Mol Cell Biol"; date: 
            "2015-04"; volume: "35"; issue: "7"; pages: "1139-56"
COMMENT     Sequence Label: pcDNA-Dest47- R5D-K6D N23 C11orf83-GFP
FEATURES             Location/Qualifiers
     source          1..757
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     53..72
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        53..71
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    104..128
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     RBS             140..160
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    complement(255..279)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(323..351)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 gcagagctct ctggctaact agagaaccca ctgcttactg gcttatcgaa attaatacga
       61 ctcactatag ggagacccaa gctggctagt taagcttgat caaacaagtt tgtacaaaaa
      121 agcaggctcc gcggccgcct tgtttaactt taagaaggag cccttcacca tggattcctt
      181 ggacgacatg ctgatctcag tcgcaatgct gggcgcaggg gctggcgtgg gctacgcgaa
      241 gggtgggcgc gccgacccag ctttcttgta caaagtggtt cgatctagaa tggctagcaa
      301 aggagaagaa cttttcactg gagttgtccc aattcttgtt gaattagatg gtgatgttaa
      361 tgggcacaaa ttttctgtca gtggagaggg tgaaggtgat gctacatacg gaaagcttac
      421 ccttaaattt atttgcacta ctggaaaact acctgttcca tggccaacac ttgtcactac
      481 tttctcttat ggtgttcaat gcttttcccg ttatccggat catatgaaac ggcatgactt
      541 tttcaagagt gccatgcccg aaggttatgt acaggaacgc actatatctt tcaaagatga
      601 cgggaactac aagacgcgtg ctgaagtcaa gtttgaaggt gatacccttg ttaatcgtat
      661 cgagttaaaa ggtattgatt ttaaagaaga tggaaacatt ctcggacaca aactcgagta
      721 caactataac tcacacaatg tatacatcac ggcagac
//
