LOCUS       sequence-117112-         764 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of C terminally V5-tagged C11orf83/UQCC3 : 
            MUTANT R5A-K6A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 764)
  AUTHORS   Desmurs M, Foti M, Raemy E, Vaz FM, Martinou JC, Bairoch A, Lane L
  TITLE     C11orf83, a mitochondrial cardiolipin-binding protein involved in 
            bc1 complex assembly and supercomplex stabilization.
  JOURNAL   Mol Cell Biol. 2015 Apr;35(7):1139-56. doi: 10.1128/MCB.01047-14. 
            Epub 2015 Jan 20.
  PUBMED    25605331
REFERENCE   2  (bases 1 to 764)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/MCB.01047-14"; journalName: "Mol Cell Biol"; date: 
            "2015-04"; volume: "35"; issue: "7"; pages: "1139-56"
COMMENT     Sequence Label: pcDNA3.2-R5A-K6A C11orf83-V5
FEATURES             Location/Qualifiers
     source          1..764
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     50..69
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        50..68
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    98..122
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     RBS             134..154
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    complement(459..483)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             509..550
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(590..609)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    634..682
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
        1 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
       61 actataggga gacccaagct ggctagttaa gctatcaaca agtttgtaca aaaaagcagg
      121 ctccgcggcc gccttgttta actttaagaa ggagcccttc accatggatt ccttggcggc
      181 aatgctgatc tcagtcgcaa tgctgggcgc aggggctggc gtgggctacg cgctcctcgt
      241 tatcgtgacc ccgggagagc ggcggaagca ggaaatgcta aaggagatgc cactgcagga
      301 cccaaggagc agggaggagg cggccaggac ccagcagcta ttgctggcca ctctgcagga
      361 ggcagcgacc acgcaggaga acgtggcctg gaggaagaac tggatggttg gcggcgaagg
      421 cggcgccggc gggaggtcac cgaagggtgg gcgcgccgac ccagctttct tgtacaaagt
      481 ggttgatcta gagggcccgc ggttcgaagg taagcctatc cctaaccctc tcctcggtct
      541 cgattctacg cgtaccggtt agtaatgagt ttaaacgggg gaggctaact gaaacacgga
      601 aggagacaat accggaagga acccgcgcta tgacggcaat aaaaagacag aataaaacgc
      661 acgggtgttg ggtcgtttgt tcataaacgc ggggttcggt cccagggctg gcactctgtc
      721 gataccccac cgagacccca ttggggccaa tacgcccgcg tttc
//
