LOCUS       sequence-117114-        1045 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of G protein-coupled receptors for PRESTO-Tango: parallel
            receptorome expression and screening via transcriptional output, 
            with transcriptional activation following arrestin translocation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1045)
  AUTHORS   Kroeze WK, Sassano MF, Huang XP, Lansu K, McCorvy JD, Giguere PM, 
            Sciaky N, Roth BL
  TITLE     PRESTO-Tango as an open-source resource for interrogation of the 
            druggable human GPCRome.
  JOURNAL   Nat Struct Mol Biol. 2015 May;22(5):362-9. doi: 10.1038/nsmb.3014. 
            Epub 2015 Apr 20.
  PUBMED    25895059
REFERENCE   2  (bases 1 to 1045)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nsmb.3014";
            journalName: "Nat Struct Mol Biol"; date: "2015-05"; volume: "22"; 
            issue: "5"; pages: "362-9"
COMMENT     Sequence Label: CHRM4-Tango
FEATURES             Location/Qualifiers
     source          1..1045
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     46..65
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        46..64
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 tctctggcta actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta
       61 tagggagacc caagctggct agcgtttaaa cttaagcttg gtaccgagct cggatccact
      121 agtccagtgt ggtggaattc tgcagatatc cagcacagtg gcggccgcgc caccatgaag
      181 acgatcatcg ccctgagcta catcttctgc ctggtattcg ccgactacaa ggacgatgat
      241 gacgccagca tcgatatggc gaatttcact cctgttaatg gttccagtgg caaccagtct
      301 gttagactgg tgacttctag ctctcacaac aggtatgaga cagttgaaat ggtcttcatt
      361 gccactgtga caggctcact gagcctggta actgtcgttg gaaacattct cgtgatgctg
      421 tccatcaaag tgaacagaca gctgcagact gtcaacaact atttcctgtt ttccctggcc
      481 tgtgctgacc tgataatagg cgctttctca atgaatctgt ataccgtgta tatcatcaaa
      541 ggttattggc ctctgggcgc cgtcgtgtgt gatttgtggt tggcactcga ctatgtggta
      601 tcaaacgctt cagtgatgaa tctgcttata atctcattcg atcgctattt ctgtgtgact
      661 aaacctctga cttacccagc ccggaggacc acaaaaatgg ccggcctcat gatcgccgcc
      721 gcatgggtcc tgtcattcgt cttgtgggca ccggctatcc tcttctggca gtttgtggtg
      781 ggcaagcgga ccgtccctga caatcagtgc ttcatccagt ttttgtcaaa tccagccgtt
      841 acttttggca cagctattgc ggctttttac ttgcccgtgg tgattatgac cgtcttgtat
      901 atccatatct cactggctag tcggagccgg gtgcataagc accggcctga agggccaaag
      961 gagaagaaag caaagacatt ggccttcctc aaaagcccct tgatgaagca atccgtgaaa
     1021 aaacctcccc cgggagaggc tgccc
//
