LOCUS       sequence-117123-         866 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  IVT vector for Fluc mRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 866)
  AUTHORS   Andries O, Cafferty SM, De Smedt SC, Weiss R, Sanders NN, Kitada T
  TITLE     N-methylpseudouridine-incorporated mRNA outperforms 
            pseudouridine-incorporated mRNA by providing enhanced protein 
            expression and reduced immunogenicity in mammalian cell lines and 
            mice.
  JOURNAL   J Control Release. 2015 Sep 2. pii: S0168-3659(15)30094-8. doi: 
            10.1016/j.jconrel.2015.08.051.
  PUBMED    26342664
REFERENCE   2  (bases 1 to 866)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Control 
            Release. 2015 Sep 2. pii: S0168-3659(15)30094-8. doi: 
            10.1016/j.jconrel.2015.08.051."
COMMENT     Sequence Label: pTK305
FEATURES             Location/Qualifiers
     source          1..866
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        53..71
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    108..132
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      133..142
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 cgcgcacatt tccccgaaaa gtgccacctg acgttaggga taacagggta attaatacga
       61 ctcactatag gatggactac gacatagtct agtccgccaa gtctagaaca agtttgtaca
      121 aaaaagcagg ctgccaccat ggaagatgcc aaaaacatta agaagggccc agcgccattc
      181 tacccactcg aagacgggac cgccggcgag cagctgcaca aagccatgaa gcgctacgcc
      241 ctggtgcccg gcaccatcgc ctttaccgac gcacatatcg aggtggacat tacctacgcc
      301 gagtacttcg agatgagcgt tcggctggca gaagctatga agcgctatgg gctgaataca
      361 aaccatcgga tcgtggtgtg cagcgagaat agcttgcagt tcttcatgcc cgtgttgggt
      421 gccctgttca tcggtgtggc tgtggcccca gctaacgaca tctacaacga gcgcgagctg
      481 ctgaacagca tgggcatcag ccagcccacc gtcgtattcg tgagcaagaa agggctgcaa
      541 aagatcctca acgtgcaaaa gaagctaccg atcatacaaa agatcatcat catggatagc
      601 aagaccgact accagggctt ccaaagcatg tacaccttcg tgacttccca tttgccaccc
      661 ggcttcaacg agtacgactt cgtgcccgag agcttcgacc gggacaaaac catcgccctg
      721 atcatgaaca gtagtggcag taccggattg cccaagggcg tagccctacc gcaccgcacc
      781 gcttgtgtcc gattcagtca tgcccgcgac cccatcttcg gcaaccagat catccccgac
      841 accgctatcc tcagcgtggt gccatt
//
