LOCUS       sequence-117142-         777 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression vector for expressing N-terminally Flag-tagged 
            short isoform of SadB.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 777)
  AUTHORS   Eklund G, Lang S, Glindre J, Ehlen A, Alvarado-Kristensson M
  TITLE     The nuclear localization of gamma-tubulin is regulated by 
            SadB-mediated phosphorylation.
  JOURNAL   J Biol Chem. 2014 Aug 1;289(31):21360-73. doi: 
            10.1074/jbc.M114.562389. Epub 2014 Jun 18.
  PUBMED    24942739
REFERENCE   2  (bases 1 to 777)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M114.562389"; journalName: "J Biol Chem"; date: 
            "2014-08-1- 1"; volume: "289"; issue: "31"; pages: "21360-73"
COMMENT     Sequence Label: pCDNA3 humanN-Flag SadBS
FEATURES             Location/Qualifiers
     source          1..777
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             74..97
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagcttacc atggattata aagatgatga tgataaaatg tcgtccgggg ccaaggaggg
      121 aggtgggggc tctcccgcct accacctccc ccacccccac ccccacccac cccagcacgc
      181 ccaatatgtg ggcccctatc ggctggagaa gacgctgggc aaaggacaga cagggctggt
      241 taaactcggg gtccactgca tcacgggtca gaaggtcgcc atcaagatcg tgaaccggga
      301 gaagctgtcg gagtcggtgc tgatgaaggt ggagcgggag atcgccatcc tgaagctcat
      361 cgaacaccca catgtcctca agctccacga cgtctacgag aacaagaaat atttgtacct
      421 ggttctggag cacgtctcgg ggggtgagct attcgactac ctggtaaaga aggggagact
      481 gacgcccaag gaggcccgaa agttcttccg ccagattgtg tctgcgctgg acttctgcca
      541 cagctactcc atctgccaca gagacctaaa gcccgagaac ctgcttttgg atgagaaaaa
      601 caacatccgc attgcagact tcggcatggc gtccctgcag gtgggggaca gcctcctgga
      661 gaccagctgc gggtcccccc attatgcgtg tccagaggtg attaaggggg aaaaatatga
      721 tggccgccgg gcagacatgt ggagctgtgg agtcatcctc ttcgccctgc tcgtggg
//
