LOCUS       sequence-117147-         877 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses V5- and SBP-tagged hnRNPK in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 877)
  TITLE     Izaurralde lab hnRNP
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 877)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pT7-V5-SBP-C1-HshnRNPK
FEATURES             Location/Qualifiers
     source          1..877
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     49..68
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        49..67
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      95..104
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             104..145
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             167..280
                     /codon_start=1
                     /product="streptavidin-binding peptide"
                     /label=SBP
                     /note="selected from a peptide library; binds streptavidin 
                     with nanomolar affinity (Keefe et al., 2001)"
                     /translation="MDEKTTGWGGGHVVEGLAGELEQLRARLEHHPQGQREP"
ORIGIN
        1 agctctctgg ctaactagag aacccactgc ttactggctt atcgaaatta atacgactca
       61 ctatagggag acccaagctc tagcgctacc ggtcgccacc atgggtaagc ctatccctaa
      121 ccctctcctc ggtctcgatt ctacgcccgg aagcggtagc ggtacgatgg acgagaagac
      181 caccggctgg gggggcggcc acgtggtgga gggcctggcc ggcgagctgg agcagctgcg
      241 ggccaggctg gagcaccacc ctcagggcca gcgggagccc agatctcgag ggatggaaac
      301 tgaacagcca gaagaaacct tccctaacac tgaaaccaat ggtgaatttg gtaaacgccc
      361 tgcagaagat atggaagagg aacaagcatt taaaagatct agaaacactg atgagatggt
      421 tgaattacgc attctgcttc agagcaagaa tgctggggca gtgattggaa aaggaggcaa
      481 gaatattaag gctctccgta cagactacaa tgccagtgtt tcagtcccag acagcagtgg
      541 ccccgagcgc atattgagta tcagtgctga tattgaaaca attggagaaa ttctgaagaa
      601 aatcatccct accttggaag agggcctgca gttgccatca cccactgcaa ccagccagct
      661 cccgctcgaa tctgatgctg tggaatgctt aaattaccaa cactataaag gaagtgactt
      721 tgactgcgag ttgaggctgt tgattcatca gagtctagca ggaggaatta ttggggtcaa
      781 aggtgctaaa atcaaagaac ttcgagagaa cactcaaacc accatcaagc ttttccagga
      841 atgctgtcct cattccactg acagagttgt tcttatt
//
