LOCUS       sequence-117152-         923 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses V5- and SBP-tagged PTB1 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 923)
  TITLE     Izaurralde lab hnRNP
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 923)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pT7-V5-SBP-C1-HsPTB1
FEATURES             Location/Qualifiers
     source          1..923
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     47..66
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        47..65
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      93..102
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             102..143
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             165..278
                     /codon_start=1
                     /product="streptavidin-binding peptide"
                     /label=SBP
                     /note="selected from a peptide library; binds streptavidin 
                     with nanomolar affinity (Keefe et al., 2001)"
                     /translation="MDEKTTGWGGGHVVEGLAGELEQLRARLEHHPQGQREP"
ORIGIN
        1 ctctctggct aactagagaa cccactgctt actggcttat cgaaattaat acgactcact
       61 atagggagac ccaagctcta gcgctaccgg tcgccaccat gggtaagcct atccctaacc
      121 ctctcctcgg tctcgattct acgcccggaa gcggtagcgg tacgatggac gagaagacca
      181 ccggctgggg gggcggccac gtggtggagg gcctggccgg cgagctggag cagctgcggg
      241 ccaggctgga gcaccaccct cagggccagc gggagcccag atctcgagac atggacggca
      301 ttgtcccaga tatagccgtt ggtacaaagc ggggatctga cgagcttttc tctacttgtg
      361 tcactaacgg accgtttatc atgagcagca actcggcttc tgcagcaaac ggaaatgaca
      421 gcaagaagtt caaaggtgac agccgaagtg caggcgtccc ctctagagtg atccacatcc
      481 ggaagctccc catcgacgtc acggaggggg aagtcatctc cctggggctg ccctttggga
      541 aggtcaccaa cctcctgatg ctgaagggga aaaaccaggc cttcatcgag atgaacacgg
      601 aggaggctgc caacaccatg gtgaactact acacctcggt gacccctgtg ctgcgcggcc
      661 agcccatcta catccagttc tctaaccaca aggagctgaa gaccgacagc tctcccaacc
      721 aggcgcgggc ccaggcggcc ctgcaggcgg tgaactcggt ccagtcgggg aacctggcct
      781 tggctgcctc ggcggcggcc gtggacgcag ggatggcgat ggccgggcag agccctgtgc
      841 tcaggatcat cgtggagaac ctcttctacc ctgtgaccct ggatgtgctg caccagattt
      901 tctccaagtt cggcacagtg ttg
//
