LOCUS       sequence-117154-         231 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Doxycycline-regulated mammalian expression vector for expressing 
            shRNA against E2F3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 231)
  AUTHORS   Hoog G, Zarrizi R, von Stedingk K, Jonsson K, Alvarado-Kristensson M
  TITLE     Nuclear localization of gamma-tubulin affects E2F transcriptional 
            activity and S-phase progression.
  JOURNAL   FASEB J. 2011 Nov;25(11):3815-27. doi: 10.1096/fj.11-187484. Epub 
            2011 Jul 25.
  PUBMED    21788450
REFERENCE   2  (bases 1 to 231)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1096/fj.11-187484"; journalName: "FASEB J"; date: "2011-11"; 
            volume: "25"; issue: "11"; pages: "3815-27"
COMMENT     Sequence Label: pTER E2F3shRNA human
FEATURES             Location/Qualifiers
     source          1..231
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     139..158
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     protein_bind    204..222
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
ORIGIN
        1 ttcgaacgct gacgtcatca acccgctcca aggaatcgcg ggcccagtgt cactaggcgg
       61 gaacacccag cgcgcgtgcg ccctggcagg aagatggctg tgagggacag gggagtggcg
      121 ccctgcaata tttgcatgtc gctatgtgtt ctgggaaatc accataaacg tgaaatgtct
      181 ttggatttgg gaatcttata agttccctat cagtgataga gatcccggct g
//
