LOCUS       sequence-117155-         799 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Doxycycline-regulated mammalian expression vector for expressing 
            shRNA against RB1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 799)
  AUTHORS   Ehlen A, Rossello CA, von Stedingk K, Hoog G, Nilsson E, Pettersson 
            HM, Jirstrom K, Alvarado-Kristensson M
  TITLE     Tumors with nonfunctional retinoblastoma protein are killed by 
            reduced gamma-tubulin levels.
  JOURNAL   J Biol Chem. 2012 May 18;287(21):17241-7. doi: 
            10.1074/jbc.M112.357038. Epub 2012 Apr 6.
  PUBMED    22493456
REFERENCE   2  (bases 1 to 799)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M112.357038"; journalName: "J Biol Chem"; date: 
            "2012-05-18- 18"; volume: "287"; issue: "21"; pages: "17241-7"
COMMENT     Sequence Label: pTER RB1shRNA human
FEATURES             Location/Qualifiers
     source          1..799
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     134..153
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     protein_bind    199..217
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     complement(388..405)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    394..618
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(751..770)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 acgctgacgt catcaacccg ctccaaggaa tcgcgggccc agtgtcacta ggcgggaaca
       61 cccagcgcgc gtgcgccctg gcaggaagat ggctgtgagg gacaggggag tggcgccctg
      121 caatatttgc atgtcgctat gtgttctggg aaatcaccat aaacgtgaaa tgtctttgga
      181 tttgggaatc ttataagttc cctatcagtg atagagatcc cgaacgatta tcattcaaat
      241 tcaagagatt tgaatggata atcgttcttt ttggaaaagc ttggtaccga gctcggatcc
      301 actagtccag tgtggtggaa ttctgcagat atccagcaca gtggcggccg ctcgagtcta
      361 gagggcccgt ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg
      421 ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt
      481 cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg
      541 gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg
      601 atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctct agggggtatc
      661 cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
      721 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
      781 ccacgttcgc cggctttcc
//
