LOCUS       sequence-117159-         768 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cre-off/Flp-on ChR2-EYFP under the short Ef1a promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 768)
  AUTHORS   Fenno LE, Mattis J, Ramakrishnan C, Hyun M, Lee SY, He M, Tucciarone
            J, Selimbeyoglu A, Berndt A, Grosenick L, Zalocusky KA, Bernstein H,
            Swanson H, Perry C, Diester I, Boyce FM, Bass CE, Neve R, Huang ZJ, 
            Deisseroth K
  TITLE     Targeting cells with single vectors using multiple-feature Boolean 
            logic.
  JOURNAL   Nat Methods. 2014 Jul;11(7):763-72. doi: 10.1038/nmeth.2996. Epub 
            2014 Jun 8.
  PUBMED    24908100
REFERENCE   2  (bases 1 to 768)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2996"; journalName: "Nat Methods"; date: "2014-07"; 
            volume: "11"; issue: "7"; pages: "763-72"
COMMENT     Sequence Label: pAAV-hnEF Coff/Fon hChR2(H134R)-EYFP
FEATURES             Location/Qualifiers
     source          1..768
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(353..386)
                     /label=loxN
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AGGTATAC) (Shaw et al., 2021). loxN sites are 
                     compatible with each other, but incompatible with loxP or 
                     lox2272 sites."
     protein_bind    complement(437..470)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
ORIGIN
        1 cctgaggatc aatgttactg tgccggatgg attgaatctc gcggcacgaa cggcgctcag
       61 accgcgtcaa atgtcctgca gtggcttgca gcaggattca gcattttgct gctgatgttc
      121 tatgcctacc aaacctggaa atctacatgc ggctgggagg agatctatgt gtgcgccatt
      181 gaaatggtta aggtgattct cgagttcttt tttgagttta agaatccctc tatgctctac
      241 cttgccacag gacaccgggt gcagtggctg cgctatgcag agtggctgct cacttgtcct
      301 gtcatcctta tccgcctgag caacctcacc ggtaagtgtc ggggttgcta gcataacttc
      361 gtatagtata ccttatacga agttattctt tgcaccattc taaagaataa cagtgataat
      421 ttctgggtta aggcaaataa cttcgtatag gatactttat acgaagttat ggtacctgtg
      481 cccccccttt tttttataaa ttgtattaat gttatataca tatctcctgt atgtgaccca
      541 tgtgcttatg actctatttc tcatgtgttt aggcctgagc aacgactaca gcaggagaac
      601 catgggactc cttgtctcag acatcgggac tatcgtgtgg ggggctacca gcgccatggc
      661 aaccggctat gttaaagtca tcttcttttg tcttggattg tgctatggcg cgaacacatt
      721 ttttcacgcc gccaaagcat atatcgaggg ttatcatact gtgccaaa
//
