LOCUS       sequence-117163-         981 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of G protein-coupled receptors for PRESTO-Tango: parallel
            receptorome expression and screening via transcriptional output, 
            with transcriptional activation following arrestin translocation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 981)
  AUTHORS   Kroeze WK, Sassano MF, Huang XP, Lansu K, McCorvy JD, Giguere PM, 
            Sciaky N, Roth BL
  TITLE     PRESTO-Tango as an open-source resource for interrogation of the 
            druggable human GPCRome.
  JOURNAL   Nat Struct Mol Biol. 2015 May;22(5):362-9. doi: 10.1038/nsmb.3014. 
            Epub 2015 Apr 20.
  PUBMED    25895059
REFERENCE   2  (bases 1 to 981)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nsmb.3014";
            journalName: "Nat Struct Mol Biol"; date: "2015-05"; volume: "22"; 
            issue: "5"; pages: "362-9"
COMMENT     Sequence Label: CHRM5-Tango
FEATURES             Location/Qualifiers
     source          1..981
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     46..65
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        46..64
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 tctctggcta actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta
       61 tagggagacc caagctggct agcgtttaaa cttaagcttg gtaccgagct cggatccact
      121 agtccagtgt ggtggaattc tgcagatatc cagcacagtg gcggccgcgc caccatgaag
      181 acgatcatcg ccctgagcta catcttctgc ctggtattcg ccgactacaa ggacgatgat
      241 gacgccagca tcgatatgga gggcgactca tatcacaatg ccacgactgt gaacggcaca
      301 cccgtcaacc accagccact tgaaaggcac aggctttggg aggtaataac catagccgca
      361 gtgactgcag tagttagtct gattaccatt gttggcaatg tattggtcat gatttctttt
      421 aaggttaaca gccagctgaa gaccgtgaat aattattatc tcctttccct ggcatgcgct
      481 gacttgatca tcgggatctt tagcatgaac ctgtatacta cgtacatcct gatggggcgc
      541 tgggcactgg gttcattggc atgtgacttg tggcttgcgc tggattatgt tgcatctaac
      601 gccagcgtaa tgaatcttct cgtgatatcc tttgacagat acttctctat tacacggccc
      661 ctgacgtaca gagccaaaag gacccccaaa cgagcaggca tcatgattgg actggcttgg
      721 ctcatatcct tcatactctg ggcccctgcg atcctctgtt ggcagtatct cgtggggaaa
      781 aggactgtgc ccctggacga atgccagatc cagttcctga gtgagcccac aataactttt
      841 gggaccgcca tagctgcttt ctatattccg gtatccgtta tgaccatcct ttactgcagg
      901 atctacagag agacagagaa aaggaccaaa gatttggctg atcttcaggg gtctgattct
      961 gtgactaaag ccgagaaaaa g
//
