LOCUS       sequence-117165-        1073 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of G protein-coupled receptors for PRESTO-Tango: parallel
            receptorome expression and screening via transcriptional output, 
            with transcriptional activation following arrestin translocation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1073)
  AUTHORS   Kroeze WK, Sassano MF, Huang XP, Lansu K, McCorvy JD, Giguere PM, 
            Sciaky N, Roth BL
  TITLE     PRESTO-Tango as an open-source resource for interrogation of the 
            druggable human GPCRome.
  JOURNAL   Nat Struct Mol Biol. 2015 May;22(5):362-9. doi: 10.1038/nsmb.3014. 
            Epub 2015 Apr 20.
  PUBMED    25895059
REFERENCE   2  (bases 1 to 1073)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nsmb.3014";
            journalName: "Nat Struct Mol Biol"; date: "2015-05"; volume: "22"; 
            issue: "5"; pages: "362-9"
COMMENT     Sequence Label: CCKAR-Tango
FEATURES             Location/Qualifiers
     source          1..1073
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     46..65
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        46..64
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 tctctggcta actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta
       61 tagggagacc caagctggct agcgtttaaa cttaagcttg gtaccgagct cggatccact
      121 agtccagtgt ggtggaattc tgcagatatc cagcacagtg gcggccgcgc caccatgaag
      181 acgatcatcg ccctgagcta catcttctgc ctggtattcg ccgactacaa ggacgatgat
      241 gacgccagca tcgatatgga cgtggttgac agtctcctcg tgaatggctc caacattacc
      301 ccaccttgcg agcttggcct cgaaaacgag acccttttct gcctggacca gccccggccc
      361 tctaaagagt ggcagcctgc cgttcagata ctgctctatt ctctgatctt cctgctttcc
      421 gttctgggaa atactttggt catcaccgtg ttgataagga acaagaggat gagaactgtg
      481 actaatatct ttctgctctc tctggctgtt agtgatttga tgctttgtct gttctgtatg
      541 ccattcaact tgatccccaa tctcttgaaa gatttcatct ttggcagcgc tgtgtgcaag
      601 acgaccacct acttcatggg cacgagtgtg tccgtcagca ctttcaacct cgttgccata
      661 tccctggagc gctatggcgc tatttgcaag cctctccaaa gtagggtgtg gcagactaag
      721 agccatgctc ttaaggtgat tgcagcaact tggtgtctga gctttacaat catgacacca
      781 tatcccattt acagtaatct ggttcccttc acaaaaaata ataaccagac cgctaacatg
      841 tgtaggttcc tcttgccaaa tgatgtcatg cagcaaagct ggcatacgtt cctgctgctc
      901 atcctgtttc tgattcccgg cattgtaatg atggttgcgt acggcctcat atctctggag
      961 ctgtatcagg gtataaaatt cgaggcgtcc cagaagaaga gtgctaaaga aaggaagcct
     1021 agcactactt ccagtggcaa atacgaggat tccgatggct gttatctgca gaa
//
