LOCUS       sequence-117168-         873 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  E. coli cloning vector (KanR, low copy, blue/white selection).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 873)
  AUTHORS   Vieira J, Messing J
  TITLE     New pUC-derived cloning vectors with different selectable markers 
            and DNA replication origins.
  JOURNAL   Gene. 1991 Apr;100:189-94.
  PUBMED    1905257
REFERENCE   2  (bases 1 to 873)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene."; 
            date: "1991-04"; volume: "100"; pages: "189-94"
COMMENT     Sequence Label: pOK12
FEATURES             Location/Qualifiers
     source          1..873
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     111..127
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     111..127
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(141..160)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(142..160)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(167..184)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(167..183)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(176..198)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 tccgaggcct cgagatctat cgatgcatgc catggtaccc gggagctcga attcgaagct
       61 tctgcagacg cgtcgacgtc atatggatcc gatatcgccg tggcggccgc tctagaacta
      121 gtggatcgat ccccaattcg ccctatagtg agtcgtatta caattcactg gccgtcgttt
      181 tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc
      241 cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt
      301 tgcgcagcct gaatggcgaa tgagcttgcg ccgtcccgtc aagtcagcgt aatgctctgc
      361 cagtgttaca accaattaac caattctgat tagaaaaact catcgagcat caaatgaaac
      421 tgcaatttat tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat
      481 gaaggagaaa actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg
      541 attccgactc gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta
      601 tcaagtgaga aatcaccatg agtgacgact gaatccggtg agaatggcaa aagtttatgc
      661 atttctttcc agacttgttc aacaggccag ccattacgct cgtcatcaaa atcactcgca
      721 tcaaccaaac cgttattcat tcgtgattgc gcctgagcga gacgaaatac gcgatcgctg
      781 ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca
      841 tcaacaatat tttcacctga atcaggatat tct
//
