LOCUS       sequence-117176-         855 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses mCherry under the control of Mutant T7 Promoter G6.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 855)
  AUTHORS   Jones JA, Vernacchio VR, Lachance DM, Lebovich M, Fu L, Shirke AN, 
            Schultz VL, Cress B, Linhardt RJ, Koffas MA
  TITLE     ePathOptimize: A Combinatorial Approach for Transcriptional 
            Balancing of Metabolic Pathways.
  JOURNAL   Sci Rep. 2015 Jun 11;5:11301. doi: 10.1038/srep11301.
  PUBMED    26062452
REFERENCE   2  (bases 1 to 855)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2015-06-11"; volume: "5:11301. doi"; pages: " 
            10.1038/srep11301"
COMMENT     Sequence Label: pETM6-G6-mCherry
FEATURES             Location/Qualifiers
     source          1..855
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    67..91
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             106..128
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 gcgtccggcg tagcctagga tcgagatcga tctcgatccc gcgaaattaa tacgactcac
       61 tatttcggaa ttgtgagcgg ataacaattc ccctctagaa ataattttgt ttaactttaa
      121 gaaggagata tacatatggt ttcaaaaggc gaagaagaca acatggcgat tatcaaggaa
      181 tttatgcgtt tcaaggtcca catggaaggc agcgtcaatg gtcacgaatt tgaaattgaa
      241 ggcgaaggtg aaggccgtcc gtatgaaggc acccagacgg caaaactgaa ggtcaccaaa
      301 ggcggtccgc tgccgtttgc ttgggatatt ctgtcaccgc aattcatgta tggttcgaaa
      361 gcgtacgtta agcatccggc cgatatcccg gactatctga aactgtcctt tccggaaggc
      421 ttcaaatggg aacgtgttat gaacttcgaa gatggcggtg tggttaccgt cacgcaggat
      481 agctctctgc aagacggtga atttatttat aaagtgaagc tgcgcggcac caatttcccg
      541 agcgatggtc cggttatgca gaaaaagacg atgggctggg aagcgagttc cgaacgtatg
      601 tacccggaag acggtgccct gaaaggcgaa atcaagcagc gcctgaaact gaaggatggc
      661 ggtcactatg acgcagaagt gaaaaccacg tacaaggcta aaaagccggt ccaactgccg
      721 ggtgcataca acgtgaacat caagctggat atcaccagcc ataacgaaga ctatacgatc
      781 gttgaacagt acgaacgtgc agaaggccgc cactctaccg gcggtatgga tgaactgtac
      841 aaataaggta ccctc
//
