LOCUS       sequence-117185-         818 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses mCherry under the control of Mutant T7 Promoter H10.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 818)
  AUTHORS   Jones JA, Vernacchio VR, Lachance DM, Lebovich M, Fu L, Shirke AN, 
            Schultz VL, Cress B, Linhardt RJ, Koffas MA
  TITLE     ePathOptimize: A Combinatorial Approach for Transcriptional 
            Balancing of Metabolic Pathways.
  JOURNAL   Sci Rep. 2015 Jun 11;5:11301. doi: 10.1038/srep11301.
  PUBMED    26062452
REFERENCE   2  (bases 1 to 818)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2015-06-11"; volume: "5:11301. doi"; pages: " 
            10.1038/srep11301"
COMMENT     Sequence Label: pETM6-H10-mCherry
FEATURES             Location/Qualifiers
     source          1..818
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    79..102
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             117..139
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 ccggccacga tgcgtccggc gtagcctagg atcgagatcg atctcgatcc cgcgaaatta
       61 atacgactca ctacggaaga attgtgagcg gataacaatt cccctctaga aataattttg
      121 tttaacttta agaaggagat atacatatgg tttcaaaagg cgaagaagac aacatggcga
      181 ttatcaagga atttatgcgt ttcaaggtcc acatggaagg cagcgtcaat ggtcacgaat
      241 ttgaaattga aggcgaaggt gaaggccgtc cgtatgaagg cacccagacg gcaaaactga
      301 aggtcaccaa aggcggtccg ctgccgtttg cttgggatat tctgtcaccg caattcatgt
      361 atggttcgaa agcgtacgtt aagcatccgg ccgatatccc ggactatctg aaactgtcct
      421 ttccggaagg cttcaaatgg gaacgtgtta tgaacttcga agatggcggt gtggttaccg
      481 tcacgcagga tagctctctg caagacggtg aatttattta taaagtgaag ctgcgcggca
      541 ccaatttccc gagcgatggt ccggttatgc agaaaaagac gatgggctgg gaagcgagtt
      601 ccgaacgtat gtacccggaa gacggtgccc tgaaaggcga aatcaagcag cgcctgaaac
      661 tgaaggatgg cggtcactat gacgcagaag tgaaaaccac gtacaaggct aaaaagccgg
      721 tccaactgcc gggtgcatac aacgtgaaca tcaagctgga tatcaccagc cataacgaag
      781 actatacgat cgttgaacag tacgaacgtg cagaaggc
//
