LOCUS       sequence-117192-        1620 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  cyan luminescent protein for high-speed single-cell and whole body 
            imaging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1620)
  AUTHORS   Takai A, Nakano M, Saito K, Haruno R, Watanabe TM, Ohyanagi T, Jin 
            T, Okada Y, Nagai T
  TITLE     Expanded palette of Nano-lanterns for real-time multicolor 
            luminescence imaging.
  JOURNAL   Proc Natl Acad Sci U S A. 2015 Apr 7;112(14):4352-6. doi: 
            10.1073/pnas.1418468112. Epub 2015 Mar 23.
  PUBMED    25831507
REFERENCE   2  (bases 1 to 1620)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1418468112"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2015-04-7- 7"; volume: "112"; issue: "14"; pages: "4352-6"
COMMENT     Sequence Label: Cyan Nano-lantern/pcDNA3
FEATURES             Location/Qualifiers
     source          1..1620
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(1..3,4..6,7..519)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYFSDNVYITADKQKNGIK
                     ANFKIRHNIE"
     primer_bind     complement(46..67)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(307..326)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     654..675
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
       61 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac
      121 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc
      181 ctcgtgacca ccctgtcctg gggcgtgcag tgcttcgccc gctaccccga ccacatgaag
      241 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc
      301 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
      361 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
      421 aagctggagt acaactactt tagcgacaac gtctatatca ccgccgacaa gcagaagaac
      481 ggcatcaagg ccaacttcaa gatccgccac aacatcgagg acggcggcgt gcagctcgcc
      541 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac
      601 tacctgagca cccagtccaa gctgagcaaa gaccccaacg agaagcgcga tcacatggtc
      661 ctgctggagt tcgtgaccgc cgccgggggt accaaggtgt acgaccccga gcagaggaag
      721 aggatgatca ccggccccca gtggtgggcc aggtgcaagc agatgaacgt gctggacagc
      781 ttcatcaact actacgacag cgagaagcac gccgagaacg ccgtgatctt cctgcacggc
      841 aacgccacta gcagctacct gtggaggcac gtggtgcccc acatcgagcc cgtggccagg
      901 tgcatcatcc ccgatctgat cggcatgggc aagagcggca agagcggcaa cggcagctac
      961 aggctgctgg accactacaa gtacctgacc gcctggttcg agcttctgaa cctgcccaag
     1021 aagatcatct tcgtgggcca cgactggggc gccgccctgg ccttccacta cgcctacgag
     1081 caccaggaca ggatcaaggc catcgtgcac atggagagcg tggtggacgt gatcgagagc
     1141 tgggacgagt ggccagacat cgaggaggac atcgccctga tcaagagcga ggagggcgag
     1201 aagatggtgc tggagaacaa cttcttcgtg gagaccgtgc tgcccagcaa gatcatgaga
     1261 aagctggagc ccgaggagtt cgccgcctac ctggagccct tcaaggagaa gggcgaggtg
     1321 agaagaccca ccctgagctg gcccagagag atccccctgg tgaagggcgg caagcccgac
     1381 gtggtgcaga tcgtgagaaa ctacaacgcc tacctgagag ccagcgacga cctgcccaag
     1441 ctgttcatcg agggcgaccc cggcttcttc agcaacgcca tcgtggaggg cgccaagaag
     1501 ttccccaaca ccgagttcgt gaaggtgaag ggcctgcact tcctccagga ggacgccccc
     1561 gacgagatgg gcaagtacat caagagcttc gtggagagag tgctgaagaa cgagcagtaa
//
