LOCUS       sequence-117206-        4863 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression vector driving dog Caveolin1-minSOG.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4863)
  AUTHORS   Ariotti N, Hall TE, Rae J, Ferguson C, McMahon KA, Martel N, Webb 
            RE, Webb RI, Teasdale RD, Parton RG
  TITLE     Modular Detection of GFP-Labeled Proteins for Rapid Screening by 
            Electron Microscopy in Cells and Organisms.
  JOURNAL   Dev Cell. 2015 Nov 23;35(4):513-25. doi: 
            10.1016/j.devcel.2015.10.016. Epub 2015 Nov 12.
  PUBMED    26585296
REFERENCE   2  (bases 1 to 4863)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2015.10.016"; journalName: "Dev Cell"; date: 
            "2015-11-23- 23"; volume: "35"; issue: "4"; pages: "513-25"
COMMENT     Sequence Label: Cav1-miniSOG
FEATURES             Location/Qualifiers
     source          1..4863
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      593..602
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             599..919
                     /codon_start=1
                     /product="mini singlet oxygen generator, a fluorescent 
                     flavoprotein suitable for correlated light and electron 
                     microscopy (Shu et al., 2011)"
                     /label=miniSOG
                     /translation="MEKSFVITDPRLPDNPIIFASDGFLELTEYSREEILGRNGRFLQG
                     PETDQATVQKIRDAIRDQREITVQLINYTKSGKKFWNLLHLQPMRDQKGELQYFIGVQL
                     DG"
     polyA_signal    1041..1162
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1078..1097)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1132..1151
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(1169..1624)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1306..1327)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1518..1537
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        1651..1755
                     /gene="bla"
                     /label=AmpR promoter
     promoter        1757..2114
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        1757..1948
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(1780..1800)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      1965..2100
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2149..2943
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             2149..2943
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2203..2222)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     2813..2832
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(3131..3150)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    3175..3222
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      3551..4139
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4040..4059
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        4314..4617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4618..4821
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4772..4792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 atctcgagct caagcttcga attctgcagt cgacggtacc accatgtctg ggggcaaata
       61 cgtagactcc gaggggcacc tctacaccgt tcccatccgg gagcagggca acatctacaa
      121 gcccaacaac aaggccatgg cggaggagat gagcgagaag caggtgtacg acgcgcacac
      181 caaggaaatc gacctggtca accgcgaccc caagcatctc aacgacgacg tggtcaagat
      241 tgattttgaa gatgtgattg cagaaccaga aggaacacac agttttgatg gcatctggaa
      301 ggccagcttc accaccttca ctgtgacaaa atactggttt taccgcttgc tgtctgccct
      361 ctttggcatc ccaatggcac tcatatgggg catttacttt gccattcttt ctttcctgca
      421 catctgggca gttgtgccgt gcattaagag tttcctgatt gagattcagt gcatcagccg
      481 tgtctattcc atctacgtcc acaccttctg tgacccgttc tttgaggctg ttggcaaaat
      541 attcagcaat atccgcatca acatgcagaa agaaacacgg gatccaccgg tcgccaccat
      601 ggagaaaagt ttcgtgataa ctgatccacg gctgccagac aatcccatca tcttcgcatc
      661 cgatggcttc ctggagctga ccgagtattc cagagaggag atcctgggcc gcaatggccg
      721 ctttctgcag ggaccagaga cagaccaggc cacagtgcag aagattcgcg atgccattag
      781 agatcagcgc gagattaccg tgcagctgat aaactacaca aaaagcggga agaaattctg
      841 gaacctcctg cacctccagc ccatgaggga ccagaagggt gagctccagt atttcatcgg
      901 agtgcagctg gatggataag cggccgcgac tctagatcat aatcagccat accacatttg
      961 tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa
     1021 tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca
     1081 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     1141 ccaaactcat caatgtatct taaggcgtaa attgtaagcg ttaatatttt gttaaaattc
     1201 gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc
     1261 ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag
     1321 agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc
     1381 gatggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa
     1441 gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg
     1501 aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt
     1561 gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc
     1621 gcgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     1681 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     1741 tgaaaaagga agagtcctga ggcggaaaga accagctgtg gaatgtgtgt cagttagggt
     1801 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
     1861 cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     1921 atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc
     1981 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
     2041 ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
     2101 taggcttttg caaagatcga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa
     2161 gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg
     2221 gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc
     2281 ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca agacgaggca
     2341 gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc
     2401 actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca
     2461 tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat
     2521 acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca
     2581 cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg
     2641 ctcgcgccag ccgaactgtt cgccaggctc aaggcgagca tgcccgacgg cgaggatctc
     2701 gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct
     2761 ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct
     2821 acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac
     2881 ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc
     2941 tgagcgggac tctggggttc gaaatgaccg accaagcgac gcccaacctg ccatcacgag
     3001 atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg
     3061 ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccctaggg
     3121 ggaggctaac tgaaacacgg aaggagacaa taccggaagg aacccgcgct atgacggcaa
     3181 taaaaagaca gaataaaacg cacggtgttg ggtcgtttgt tcataaacgc ggggttcggt
     3241 cccagggctg gcactctgtc gataccccac cgagacccca ttggggccaa tacgcccgcg
     3301 tttcttcctt ttccccaccc caccccccaa gttcgggtga aggcccaggg ctcgcagcca
     3361 acgtcggggc ggcaggccct gccatagcct caggttactc atatatactt tagattgatt
     3421 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     3481 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     3541 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     3601 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     3661 taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag
     3721 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     3781 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     3841 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     3901 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     3961 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     4021 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     4081 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     4141 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     4201 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccatg cattagttat
     4261 taatagtaat caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca
     4321 taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca
     4381 ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg
     4441 gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg
     4501 ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc
     4561 ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg
     4621 atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca
     4681 agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt
     4741 ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg
     4801 gaggtctata taagcagagc tggtttagtg aaccgtcaga tccgctagcg ctaccggact
     4861 cag
//
