LOCUS       sequence-117212-       12281 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Zebrafish expression vector for ubiquitous expression of APEX2-GBP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12281)
  AUTHORS   Ariotti N, Hall TE, Rae J, Ferguson C, McMahon KA, Martel N, Webb 
            RE, Webb RI, Teasdale RD, Parton RG
  TITLE     Modular Detection of GFP-Labeled Proteins for Rapid Screening by 
            Electron Microscopy in Cells and Organisms.
  JOURNAL   Dev Cell. 2015 Nov 23;35(4):513-25. doi: 
            10.1016/j.devcel.2015.10.016. Epub 2015 Nov 12.
  PUBMED    26585296
REFERENCE   2  (bases 1 to 12281)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2015.10.016"; journalName: "Dev Cell"; date: 
            "2015-11-23- 23"; volume: "35"; issue: "4"; pages: "513-25"
COMMENT     Sequence Label: Bact2-APEX2-GBP in pDEST-Tol2-pA2-acrys-cherry
FEATURES             Location/Qualifiers
     source          1..12281
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(144..165)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    join(514..525,526..688)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     polyA_signal    1057..1178
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(1301..2011)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEDDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGHHSTGGMDELYK"
     primer_bind     complement(1381..1404)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(1579..1598)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     1848..1866
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     regulatory      2008..2017
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     2659..2675
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2695..2715
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    8022..8046
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      8047..8056
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             8053..8802
                     /codon_start=1
                     /product="soybean ascorbate peroxidase, engineered to serve
                     as a monomeric tag for electron microscopy and live-cell 
                     proteomics (Lam et al., 2015)"
                     /label=APEX2
                     /note="more active than the original APEX due to the A134P 
                     mutation"
                     /translation="MGKSYPTVSADYQDAVEKAKKKLRGFIAEKRCAPLMLRLAFHSAG
                     TFDKGTKTGGPFGTIKHPAELAHSANNGLDIAVRLLEPLKAEFPILSYADFYQLAGVVA
                     VEVTGGPKVPFHPGREDKPEPPPEGRLPDPTKGSDHLRDVFGKAMGLTDQDIVALSGGH
                     TIGAAHKERSGFEGPWTSNPLIFDNSYFTELLSGEKEGLLQLPSDKALLSDPVFRPLVD
                     KYAADEDAFFADYAEAHQKLSELGFADA"
     protein_bind    complement(8804..8828)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             8833..9174
                     /codon_start=1
                     /product="GFP-binding fragment of a single-chain camelid 
                     antibody (Rothbauer et al., 2008)"
                     /label=GFP nanobody
                     /translation="VQLVESGGALVQPGGSLRLSCAASGFPVNRYSMRWYRQAPGKERE
                     WVAGMSSAGDRSSYEDSVKGRFTISRDDARNTVYLQMNSLKPEDTAVYYCNVNVGFEYW
                     GQGTQVTVSS"
     protein_bind    complement(9178..9198)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(9206..9222)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    9238..9372
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    join(9811..9998,9999..10010)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(10044..10060)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10044..10060)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(10097..10117)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(10097..10115)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(10136..10152)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10149..10171)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10160..10176
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10184..10190,10191..10208,10209..10214))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10229..10250
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10367..10384)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10538..11126)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10618..10637)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11297..12088,12089..12157))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11920..11939
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12158..12262)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
       61 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
      121 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
      181 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacgc tggctaagaa
      241 ctcatcagcc tccccggtcc atctacccac gtaccaatgc accaattggc cacaatgacg
      301 gctactacat ggtgccattc cttcctcttt ataggaatgg agactacctc ctgtccaaca
      361 aggctcttgg atacgagtac gcctacctgt tggacccagg tcattgcaca acaccagaaa
      421 tgccctctga tctgcaaaag acgtgaatat ctgttcagac acccatatcc actctgttcc
      481 acacaggtca gaggtttgtc caggagttct tgacagaggt gtaaaaagta ctcaaaaatt
      541 ttactcaagt gaaagtacaa gtacttaggg aaaattttac tcaattaaaa gtaaaagtat
      601 ctggctagaa tcttacttga gtaaaagtaa aaaagtactc cattaaaatt gtacttgagt
      661 attaaggaag taaaagtaaa agcaagaaag aaaactagag attcttgttt aagcttttaa
      721 tctcaaaaaa cattaaatga aatgcataca aggttttatc ctgctttaga actgtttgta
      781 tttaattatc aaactataag acagacaatc taatgccagt acacgctact caaagttgta
      841 aaacctcaga tttaacttca gtagaagctg attctcaaaa ttgttagtgt caagcctagc
      901 tcttttgggg ctgaaaagca atcctgcagt gctgaaaagc ctctcacagg cagccgatgc
      961 gggaagaggt gtattagtct tgatagagag gctgcaaata gcaggaaacg tgagcagaga
     1021 ctccctggtg tctgaaacac aggcccattt acgccttaag atacattgat gagtttggac
     1081 aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg
     1141 ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt
     1201 ttatgtttca ggttcagggg gaggtgtggg aggtttttta aagcaagtaa aacctctaca
     1261 aatgtggtat ggctgattat gatctagagt cgcggccgct ttacttgtac agctcgtcca
     1321 tgccgccggt ggagtggtgg ccctcggcgc gctcgtactg ttccacgatg gtgtagtcct
     1381 cgttgtggga ggtgatgtcc agcttgatgt tgacgttgta ggcgccgggc agctgcacgg
     1441 gcttcttggc cttgtaggtg gtcttgacct cggcgtcgta gtggccgccg tccttcagct
     1501 tcagcctctg cttgatctcg cccttcaggg cgccgtcctc ggggtacatc cgctcggagg
     1561 aggcctccca gcccatggtc ttcttctgca ttacggggcc gtcggagggg aagttggtgc
     1621 cgcgcagctt caccttgtag atgaactcgc cgtcctgcag ggaggagtcc tgggtcacgg
     1681 tcaccacgcc gccgtcctcg aagttcatca cgcgctccca cttgaagccc tcggggaagg
     1741 acagcttcaa gtagtcgggg atgtcggcgg ggtgcttcac gtaggccttg gagccgtaca
     1801 tgaactgagg ggacaggatg tcccaggcga agggcagggg gccgcccttg gtcaccttca
     1861 gcttggcggt ctgggtgccc tcgtaggggc ggccctcgcc ctcgccctcg atctcgaact
     1921 cgtggccgtt cacggagccc tccatgtgca ccttgaagcg catgaactcc ttgatgatgg
     1981 ccatgttgtc atcctcgccc ttgctcacca tggtggcgac cggtaatgtc agacctggta
     2041 actccttact gtaacttatt gcacctgatg aacaaaagat gtgaactgaa cgcaagtagg
     2101 aggctaatag aggctagtac agtacaaaga tccccaaatg atgtgtgccg gccttagtgg
     2161 aggcagaccc ctggttatat acctgtgctg cagaaagagg ggcttacaaa tggatctctc
     2221 tggaatggca ttatctcatg gtgggaacag tgttgtcagc agaatcagcg gggtggactc
     2281 tgacctgaga atgacagtct agcactgaat ggctcagaca atactcttgt ggccaactat
     2341 caccggaaaa taagtgcctg ccaaaacaaa ccagaccaga tgaagctaca tggctgtaga
     2401 acgtcagtat ttaatgtgtt gtggccactt ggttcaccag atttaagagc taattttttt
     2461 aaatatcaca gataatcaat cgcttttttt tttcttgact gaaaagctta gactggaaaa
     2521 ttctttgaaa aagtttgcac tacagaaaat aaatacataa catcctgcac tgaatgcaca
     2581 ctattaatag aaaagagagc attattgtag gaaatgtatt gtatatgtac attctgtgca
     2641 tttcacaatt ttctcgagca ggaaacagct atgaccatga ttacgccaag ctatcaactt
     2701 tgtatagaaa agttgaaaat tccagtttga agaaactttt caagactcac cttttcactg
     2761 ttggtcttaa tttaggttga ttatttttgt caatttaata attacattgt attgttcata
     2821 ttttattgtc ttattgtggt gtttttacag cttttttatt gttctgcttt gttactgttt
     2881 gccttgtatg cattgtagcg ctttgggtct gagaaaagcg cactataaat aaactgtatt
     2941 attattatta gggatggaca tcacggtggc gcgatgggtg gcacaattac ctcacagcaa
     3001 gtaggttgct gtttagagcc ccggctggat cagttggcag ttctgtgtgg agtttgcatg
     3061 ttctccctgt tcaagtaggt ttcctcccgg tgctctggtt tcccctcaca agtccaaaga
     3121 catgatgtat aggtgaatgg ggtaagctaa attgaccata gtgagtgtgt gtgtgtgtgt
     3181 gaatgagagt gtataggtgt ttcccagtgt taggttgcaa ctggaaggac atctgctgtg
     3241 taaaacatat gcaggataag ttggcggttc attcccctat ggtgacccct ggttaattaa
     3301 aggactaagt tgaaaatgaa tgaatgaatg aatgattaga gataaaccga taccactttt
     3361 ttacaaaccg atatgagtac aagtataata gtttctgtta ctcgacaata ctgagtaccg
     3421 attctgatac ttcttggatt tggtttcaat gtaattaatt tgaaagaagg ttttatttct
     3481 tgcctgtcgc aaggatgaac acaaaaatct ttaccagaag gatattttaa gctaagaata
     3541 tacgcacgtt gttgacaacc ctgtatcgat taccgattaa acgtggacat ttaaaggata
     3601 agttatgctt gcgccatcac aactttacac attacatgat caaagatcat attgaatatt
     3661 cggtttattc gtcagaagcc tatagatttg ttaaaagtgt attttataaa cggatggttt
     3721 ataaagacta agctattaat atatcagaca ttcattaata catcaagaca tcgatgtcag
     3781 gtaggacaac aaatcacatc catgtaatga aatcttgttg agtggtattg gtgcgtggtt
     3841 tcagcatttc atgtgcaata cgagtaagaa tattttaacc aagtatcgac ccaaaacctg
     3901 atactggtat cggtgcatcc cttattatta ttattatcaa aaacacactt gttttattta
     3961 tgtgggtgcc aaacaacaaa ctatgaaatg cagcatgaca cgcccaaaaa gtgttccttt
     4021 gagaacaact tcataaatat gtttcttatc attatgcatg aatggtgtac tgggagagct
     4081 ttggtatcag aattgagata agagactttg gcattctaaa ggacaatgac attatcacac
     4141 tgacagtcaa caacacttta ttgctctgga aatcttatgt catggggcaa agctgaggtt
     4201 cattaggaca gtaccttatt tagagatgaa tgggaaggct gtagtgggct gctatcaggg
     4261 aagggtggtg gtcctcgggg gaactatgga ctcacaaaag cacatctgct gtaacaagat
     4321 cccctgtgca atctacaaaa gttacaaaga gaagcatggt tggttaaaaa atagaattga
     4381 ctggatatgc tgaatgcatt gtttgttgtg tgttacatca cattaaccac agattggtgc
     4441 taaatatttg cttatcttat aaaaacctgt gtgtttataa aggggggtgt ggatggatgc
     4501 ttggcattct ttgaaaagat gttgggggat ggaaggagga gaccttattg atgagtatcc
     4561 agtttgcaga taacaagttt ttagacattc atactatgat gctgtttatg tctgtttttt
     4621 ctcatttttt ctatacacaa acagctataa aacattcact tttatatctg atgctgtata
     4681 ttaaatttcg ttgtatttat tctgtttgct ctgttgggtc atagctaggg ccagacagaa
     4741 tctgctgaca ttttttgctt tttctgcaga gaattttgta aacaatctgt gatttatgcg
     4801 agattttttc gggagtatcg taactaaaaa cttactatat gaaataaaaa aaaataatac
     4861 ctttttaact tttaataaat atatacaatg caagtccacc tagatccaat tatttggtcg
     4921 acaaagcaag tctctcacat aatatatctt ctaaaagaca gaaaacatta ctttcagacc
     4981 atattgtaat caaatgaaca ctttcatatt agtcaataat attacggaaa tgtatttaaa
     5041 aactgaataa acatatattt acacctttgt ctttatttct agagtgcttt tacaatgtag
     5101 attgtgtcaa agcagcttaa cttagaggat tagtatagta aattgaaact gtgtcagtcc
     5161 attttttaag agtttaagtt cagttcagtt tagttcagtg tgagttaatt tttactgctg
     5221 agagtccaaa cactaaagag caaatccaaa tacacaagtc aataaataaa ctcaatgatg
     5281 ggctaaagat ctggggaaat ctgcagattt ctgtgcgcac agattctgtg tgggcctagt
     5341 cattacctat actcaacgtt tactctgaga aacttccata ttttagaggt aaaatgctca
     5401 gaattgtaaa aagtcttgtc aaaatacttg gaatttgcca aaaacaaaat aaaagtcaca
     5461 tcacctcatt gtccttggta tcagcaagca cttttaacac aatcagctgt ttttgtttat
     5521 aactcccact gaaggccatg tgcattactc ccaagttaca cagtaaaaag tcacatgtag
     5581 tttatcatga tcactgctgc tcaagcacag ctttatctgt tattcagtgt ggcaatatct
     5641 gaaatgtgtg caaaatatta tttattgtag ctgatcacca acactttcag tgtttttttt
     5701 ttctactttt aatgagaatt atgttcttag gatttatcct tataaacctc tttaaaaaga
     5761 aatgttatgt ctgcatagac acgacatgaa attatgaaca gaaaatttaa ctatttaaga
     5821 tcaagacttg acatataaca tttctaatga acattaaacc tgaggtaggc ccacttgcaa
     5881 aaatgtaact gctatcactc actttataca tcattgtgta acttgtacac acaaaaatag
     5941 tgaagcaatg tggcatggaa tgcaggtccc attcattcaa agttattaaa gcactttctt
     6001 aaagtgcatt actactgagc taagaccagc tagttgctca tacaacatac tacaagtagt
     6061 gtcatcctaa tttggataaa aaaaatgtcc attttataca tggaaaagta ctcattagat
     6121 cccccattgt ttgtattacg gtatttcgtg aacacaagag gtaaatgacc tacagagctg
     6181 ctgttgtgtt agattgaaaa cacaacacag gatcatggag gcatccatca ttatgacgca
     6241 ttctgccact tgtaactctg cacagacttt ttgaaaagtt taattgagtc ttggcgttgt
     6301 cgcagaaaaa tacgaaaaat tctgtgttaa aatttgcaaa taaaaattcc agttttatag
     6361 gaaatcgcca tggccttgtt ctttaaccag ttaagcttcc ccttctttca ctctcaagtt
     6421 gcaagaagca agtgtagcaa tgtgcacgcg acagccgggt gtgtgacgct ggaccaatca
     6481 gagcacagag ctccgaaagt ttacctttta tggctagagc cgggtatgtg ccgtcatata
     6541 aaagagcgcg cccagctttt cagcctcact ttgagctcct ccacacgcag ctagtgcgga
     6601 atatcatctg cttgtaaccc attctcttaa gtcgacaacc ccccaaaccc aaggtgagtt
     6661 ggttttacag tttttaagcg tttaattata gtttctatct attaatgtta agtaaaatgt
     6721 gtaaatggat aaatatgatt tgatgaagtc tttagacagt tcataacggt ctctttgtag
     6781 ttatctacta ggtttgattt ataagtggtg gttcttttga taatctatgt tacatttaat
     6841 aaatattggc tgtatcttaa cttttttgta gcgttagtgg atgtggcagg tgagaatgtc
     6901 ggtaatttaa cgtgaccagt cgtaggcacg acattgaaca ttgaataggc cggtgtgaaa
     6961 tgggtgttaa gtcttaatat aaagttgtgt tctggctaat ctctctttaa cagccataaa
     7021 tgtgattttt aactacagtt ttgagggtgc tggtgagcat gttgcacact tgatggatag
     7081 ccggcatggg aagttctttg tgcaggcagt gctgcagcag ggtgtgacct actttagcta
     7141 gccggctaac cagctctcat ctgatgtaac gtaaacccca tcaatctaac atcggctcat
     7201 tcgtgcttta taagatcgaa gatcttgtta ttagtaacgg gtattggcat ttagttttaa
     7261 cttggttaat tttctttttg ttatgtgttc tatttaaatg tagcctgcag ccccctctgt
     7321 ctttaatgaa gctgaagatt aacgttaacg ttagtgaagt gttgtatccg ctatgaaaga
     7381 tttgaatttt agaggtgtgg tccactttaa taacggctgc ggagttctct ggctgcactt
     7441 cagccgctgt gactgtcgaa gcagagccca tcagtttttc aggatctcgg tgatttttga
     7501 ggctagacta tgaactgaac cgactgagag tgaccgtcca gaaatgcttt agtgtgaagt
     7561 ctccgctcac gaggagtccg tggactgact gcagatctgt gacgcagtaa cacccggcgg
     7621 cagacacccg ttgaaatgcg gttgtgtaat tgatcgcagg cgagggtgga agaggatgtg
     7681 aaacttcatt tgtgtaaaat ttagggagtg gtcctggtgt gatgaatgtc gaaatctgtt
     7741 cctttttact gagccctacg actctggctg agtgccacac cgccggcagc cgcaaagcgg
     7801 ctcaaaccat tgccttttat ggtaataatg agagaatgca gagggacttc ctttgtctgg
     7861 catatctgag gcgcgcgctg tcactagcgc ccaccagcgg tcagactgta gaatgcagag
     7921 caaaacagga agttgactcc agatggtcac atgcttgctg aaacgcctct ccctggcagc
     7981 agtgcaattc taacagtttc cctttctttt acagttcagc ctcaagtttg tacaaaaaag
     8041 caggctgcca ccatgggaaa gtcttaccca actgtgagtg ctgattacca ggacgccgtt
     8101 gagaaggcga agaagaagct cagaggcttc atcgctgaga agagatgcgc tcctctaatg
     8161 ctccgtttgg cattccactc tgctggaacc tttgacaagg gcacgaagac cggtggaccc
     8221 ttcggaacca tcaagcaccc tgccgaactg gctcacagcg ctaacaacgg tcttgacatc
     8281 gctgttaggc ttttggagcc actcaaggcg gagttcccta ttttgagcta cgccgatttc
     8341 taccagttgg ctggcgttgt tgccgttgag gtcacgggtg gacctaaggt tccattccac
     8401 cctggaagag aggacaagcc tgagccacca ccagagggtc gcttgcccga tcccactaag
     8461 ggttctgacc atttgagaga tgtgtttggc aaagctatgg ggcttactga ccaagatatc
     8521 gttgctctat ctgggggtca cactattgga gctgcacaca aggagcgttc tggatttgag
     8581 ggtccctgga cctctaatcc tcttattttc gacaactcat acttcacgga gttgttgagt
     8641 ggtgagaagg aaggtctcct tcagctacct tctgacaagg ctcttttgtc tgaccctgta
     8701 ttccgccctc tcgttgacaa atatgcagcg gacgaagatg ccttctttgc tgattacgct
     8761 gaggctcacc aaaagctttc cgagcttggg tttgctgatg ccgacccagc tttcttgtac
     8821 aaagtggtgc aggtgcagct ggtggagtct ggaggagctc tggtgcagcc tggaggaagc
     8881 ctgcgcctga gctgtgcagc tagcggattt cctgtgaacc gctacagcat gcgctggtac
     8941 cgccaggctc ctggtaaaga gcgcgagtgg gtggctggaa tgagcagcgc tggagatcgc
     9001 agcagctacg aggacagcgt gaaaggacgc tttacaatca gccgcgatga tgctcgcaac
     9061 acagtgtacc tgcagatgaa ctctctgaaa cctgaggaca ctgctgtgta ctactgtaac
     9121 gtgaacgtgg gtttcgagta ctggggacag ggaacacagg tgacagtgag ctcttgacaa
     9181 ctttattata catagttgat aattcactgg ccgtcgtttt acggtaccat cgatgatgat
     9241 ccagacatga taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa
     9301 aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc
     9361 aataaacaag ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggaggtg
     9421 tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg gtatggctga ttatgatcct
     9481 ctagatcaga tctgcgaaga tacggccacg ggtgctcttg atcctgtggc tgattttgga
     9541 ctgtgctgct cgcagctgct gatgaatcac atacttcctc cattttcttc cactgattga
     9601 ctgttataat ttccctaatt tccaggtcaa ggtgctgtgc attgtggtaa tagatgtgac
     9661 atgacgtcac ttccaaagga ccaatgaaca tgtctgacca atttcatata atgtgaaaac
     9721 gattttcata ggcagaataa ataacattta aattaaactg ggcatcagcg caattcaatt
     9781 ggtttggtaa tagcaaggga aaatagaatg aagtgatctc caaaaaataa gtactttttg
     9841 actgtaaata aaattgtaag gagtaaaaag tacttttttt tctaaaaaaa tgtaattaag
     9901 taaaagtaaa agtattgatt tttaattgta ctcaagtaaa gtaaaaatcc ccaaaaataa
     9961 tacttaagta cagtaatcaa gtaaaattac tcaagtactt tacacctctg gttcttgacc
    10021 ccctaccttc agcaagccca gcagatccac tagttctaga gcggccgcca ccgcggtgga
    10081 gctccagctt ttgttccctt tagtgagggt taattgcgcg cttggcgtaa tcatggtcat
    10141 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
    10201 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
    10261 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
    10321 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
    10381 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
    10441 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
    10501 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
    10561 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
    10621 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
    10681 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
    10741 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
    10801 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
    10861 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
    10921 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga
    10981 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
    11041 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
    11101 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
    11161 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
    11221 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
    11281 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
    11341 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
    11401 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
    11461 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
    11521 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
    11581 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
    11641 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
    11701 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
    11761 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
    11821 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
    11881 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
    11941 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
    12001 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
    12061 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
    12121 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
    12181 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
    12241 ataaacaaat aggggttccg cgcacatttc cccgaaaagt g
//
