LOCUS       sequence-117223-         806 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Doxycycline-regulated mammalian expression vector for expressing 
            shRNA against E2F1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 806)
  AUTHORS   Hoog G, Zarrizi R, von Stedingk K, Jonsson K, Alvarado-Kristensson M
  TITLE     Nuclear localization of gamma-tubulin affects E2F transcriptional 
            activity and S-phase progression.
  JOURNAL   FASEB J. 2011 Nov;25(11):3815-27. doi: 10.1096/fj.11-187484. Epub 
            2011 Jul 25.
  PUBMED    21788450
REFERENCE   2  (bases 1 to 806)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1096/fj.11-187484"; journalName: "FASEB J"; date: "2011-11"; 
            volume: "25"; issue: "11"; pages: "3815-27"
COMMENT     Sequence Label: pTER E2F1shRNA human
FEATURES             Location/Qualifiers
     source          1..806
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     139..158
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     protein_bind    204..222
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     complement(394..411)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    400..624
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(757..776)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ttcgaacgct gacgtcatca acccgctcca aggaatcgcg ggcccagtgt cactaggcgg
       61 gaacacccag cgcgcgtgcg ccctggcagg aagatggctg tgagggacag gggagtggcg
      121 ccctgcaata tttgcatgtc gctatgtgtt ctgggaaatc accataaacg tgaaatgtct
      181 ttggatttgg gaatcttata agttccctat cagtgataga gatccctatc tgtactacgc
      241 agctgttcaa gagacagctg cgtagtacag atatttttgg aaaagcttgg taccgagctc
      301 ggatccacta gtccagtgtg gtggaattct gcagatatcc agcacagtgg cggccgctcg
      361 agtctagagg gcccgtttaa acccgctgat cagcctcgac tgtgccttct agttgccagc
      421 catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg
      481 tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc
      541 tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg
      601 ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctctaggg
      661 ggtatcccca cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
      721 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct
      781 ttctcgccac gttcgccggc tttccc
//
