LOCUS       sequence-117230-         855 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses mCherry under the control of Mutant T7 Promoter H9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 855)
  AUTHORS   Jones JA, Vernacchio VR, Lachance DM, Lebovich M, Fu L, Shirke AN, 
            Schultz VL, Cress B, Linhardt RJ, Koffas MA
  TITLE     ePathOptimize: A Combinatorial Approach for Transcriptional 
            Balancing of Metabolic Pathways.
  JOURNAL   Sci Rep. 2015 Jun 11;5:11301. doi: 10.1038/srep11301.
  PUBMED    26062452
REFERENCE   2  (bases 1 to 855)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2015-06-11"; volume: "5:11301. doi"; pages: " 
            10.1038/srep11301"
COMMENT     Sequence Label: pETM6-H9-mCherry
FEATURES             Location/Qualifiers
     source          1..855
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    70..93
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             108..130
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 atgcgtccgg cgtagcctag gatcgagatc gatctcgatc ccgcgaaatt aatacgactc
       61 actaatactg aattgtgagc ggataacaat tcccctctag aaataatttt gtttaacttt
      121 aagaaggaga tatacatatg gtttcaaaag gcgaagaaga caacatggcg attatcaagg
      181 aatttatgcg tttcaaggtc cacatggaag gcagcgtcaa tggtcacgaa tttgaaattg
      241 aaggcgaagg tgaaggccgt ccgtatgaag gcacccagac ggcaaaactg aaggtcacca
      301 aaggcggtcc gctgccgttt gcttgggata ttctgtcacc gcaattcatg tatggttcga
      361 aagcgtacgt taagcatccg gccgatatcc cggactatct gaaactgtcc tttccggaag
      421 gcttcaaatg ggaacgtgtt atgaacttcg aagatggcgg tgtggttacc gtcacgcagg
      481 atagctctct gcaagacggt gaatttattt ataaagtgaa gctgcgcggc accaatttcc
      541 cgagcgatgg tccggttatg cagaaaaaga cgatgggctg ggaagcgagt tccgaacgta
      601 tgtacccgga agacggtgcc ctgaaaggcg aaatcaagca gcgcctgaaa ctgaaggatg
      661 gcggtcacta tgacgcagaa gtgaaaacca cgtacaaggc taaaaagccg gtccaactgc
      721 cgggtgcata caacgtgaac atcaagctgg atatcaccag ccataacgaa gactatacga
      781 tcgttgaaca gtacgaacgt gcagaaggcc gccactctac cggcggtatg gatgaactgt
      841 acaaataagg taccc
//
