LOCUS       sequence-117235-         882 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Parent vector used for inserting cis-regulatory elements (CREs) for 
            CRE-seq in ES cells. Uses the Pou5f1 basal promoter and dsRed gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 882)
  AUTHORS   Fiore C, Cohen BA
  TITLE     Interactions between pluripotency factors specify cis-regulation in 
            embryonic stem cells.
  JOURNAL   Genome Res. 2016 Jun;26(6):778-86. doi: 10.1101/gr.200733.115. Epub 
            2016 Apr 15.
  PUBMED    27197208
REFERENCE   2  (bases 1 to 882)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1101/gr.200733.115"; journalName: "Genome Res"; date: "2016-06";
            volume: "26"; issue: "6"; pages: "778-86"
COMMENT     Sequence Label: pGL-Pou
FEATURES             Location/Qualifiers
     source          1..882
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(675..695)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
ORIGIN
        1 actggccggt acctgacctc gctagcgtcg aggacatcaa gatctggcct cggggcccaa
       61 gcttattggc acacgaacat tcaatggatg ttttaggctc tccagaggat ggctgagtgg
      121 gctgtaagga caggccgaga gggtgcagtg ccaacaggct ttgtggtgcg atggggcatc
      181 cgagcaactg gtttgtgagg tgtccggtga cccaaggcag gggtgagagg accttgaagg
      241 ttgaaaatga aggcctcctg gggtcccgtc ctaagggttg tcctgtccag acgtccccaa
      301 cctccgtctg gaagacacag gcagatagcg ctcgcctcag tttctcccac ccccacagct
      361 ctgctcctcc acccacccag ggggcggggc cagaggtcaa ggctagaggg tgggattggg
      421 gagggagagg tgaaaccgtc cctaggtgag ccgtctttcc accaggcccc cggctcgggg
      481 tgcccacctt ccccatggat agcactgaga acgtcatcaa gcccttcatg cgcttcaagg
      541 tgcacatgga gggctccgtg aacggccacg agttcgagat cgagggcgag ggcgagggca
      601 agccctacga gggcacccag accgccaagc tgcaggtgac caagggcggc cccctgccct
      661 tcgcctggga catcctgtcc ccccagttcc agtacggctc caaggtgtac gtgaagcacc
      721 ccgccgacat ccccgactac aagaagctgt ccttccccga gggcttcaag tgggagcgcg
      781 tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc ctgcaggacg
      841 gcaccttcat ctaccacgtg aagttcatcg gcgtgaactt cc
//
