LOCUS       sequence-117236-        4421 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Luciferase-based reporter for VEGF promoter region (-950 to -700 
            bp).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4421)
  AUTHORS   Wood LW, Cox NI, Phelps CA, Lai SC, Poddar A, Talbot C Jr, Mu D
  TITLE     Thyroid Transcription Factor 1 Reprograms Angiogenic Activities of 
            Secretome.
  JOURNAL   Sci Rep. 2016 Feb 25;6:19857. doi: 10.1038/srep19857.
  PUBMED    26912193
REFERENCE   2  (bases 1 to 4421)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2016-02-25"; volume: "6:19857. doi"; pages: " 
            10.1038/srep19857"
COMMENT     Sequence Label: pGL4.10-VEGFprom(-950 to -700)
FEATURES             Location/Qualifiers
     source          1..4421
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      273..282
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             279..1931
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     polyA_signal    1975..2096
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1986..2005)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2040..2059
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(2344..2361)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2515..3103)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2595..2614)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3303..4094,4095..4163))
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     polyA_signal    4268..4316
                     /note="synthetic polyadenylation signal"
     misc_feature    4330..4421
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     4370..4389
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
ORIGIN
        1 ggcctaactg gccggtacca agcccattcc ctctttagcc agagccgggg tgtgcagacg
       61 gcagtcacta gggggcgctc ggccaccaca gggaagctgg gtgaatggag cgagcagcgt
      121 cttcgagagt gaggacgtgt gtgtctgtgt gggtgagtga gtgtgtgcgt gtggggttga
      181 gggcgttgga gcggggagaa ggccaggggt cactccagga ttccaataga tctgtgtgtc
      241 cctaagcttg gcaatccggt actgttggta aagccaccat ggaagatgcc aaaaacatta
      301 agaagggccc agcgccattc tacccactcg aagacgggac cgccggcgag cagctgcaca
      361 aagccatgaa gcgctacgcc ctggtgcccg gcaccatcgc ctttaccgac gcacatatcg
      421 aggtggacat tacctacgcc gagtacttcg agatgagcgt tcggctggca gaagctatga
      481 agcgctatgg gctgaataca aaccatcgga tcgtggtgtg cagcgagaat agcttgcagt
      541 tcttcatgcc cgtgttgggt gccctgttca tcggtgtggc tgtggcccca gctaacgaca
      601 tctacaacga gcgcgagctg ctgaacagca tgggcatcag ccagcccacc gtcgtattcg
      661 tgagcaagaa agggctgcaa aagatcctca acgtgcaaaa gaagctaccg atcatacaaa
      721 agatcatcat catggatagc aagaccgact accagggctt ccaaagcatg tacaccttcg
      781 tgacttccca tttgccaccc ggcttcaacg agtacgactt cgtgcccgag agcttcgacc
      841 gggacaaaac catcgccctg atcatgaaca gtagtggcag taccggattg cccaagggcg
      901 tagccctacc gcaccgcacc gcttgtgtcc gattcagtca tgcccgcgac cccatcttcg
      961 gcaaccagat catccccgac accgctatcc tcagcgtggt gccatttcac cacggcttcg
     1021 gcatgttcac cacgctgggc tacttgatct gcggctttcg ggtcgtgctc atgtaccgct
     1081 tcgaggagga gctattcttg cgcagcttgc aagactataa gattcaatct gccctgctgg
     1141 tgcccacact atttagcttc ttcgctaaga gcactctcat cgacaagtac gacctaagca
     1201 acttgcacga gatcgccagc ggcggggcgc cgctcagcaa ggaggtaggt gaggccgtgg
     1261 ccaaacgctt ccacctacca ggcatccgcc agggctacgg cctgacagaa acaaccagcg
     1321 ccattctgat cacccccgaa ggggacgaca agcctggcgc agtaggcaag gtggtgccct
     1381 tcttcgaggc taaggtggtg gacttggaca ccggtaagac actgggtgtg aaccagcgcg
     1441 gcgagctgtg cgtccgtggc cccatgatca tgagcggcta cgttaacaac cccgaggcta
     1501 caaacgctct catcgacaag gacggctggc tgcacagcgg cgacatcgcc tactgggacg
     1561 aggacgagca cttcttcatc gtggaccggc tgaagagcct gatcaaatac aagggctacc
     1621 aggtagcccc agccgaactg gagagcatcc tgctgcaaca ccccaacatc ttcgacgccg
     1681 gggtcgccgg cctgcccgac gacgatgccg gcgagctgcc cgccgcagtc gtcgtgctgg
     1741 aacacggtaa aaccatgacc gagaaggaga tcgtggacta tgtggccagc caggttacaa
     1801 ccgccaagaa gctgcgcggt ggtgttgtgt tcgtggacga ggtgcctaaa ggactgaccg
     1861 gcaagttgga cgcccgcaag atccgcgaga ttctcattaa ggccaagaag ggcggcaaga
     1921 tcgccgtgta ataattctag agtcggggcg gccggccgct tcgagcagac atgataagat
     1981 acattgatga gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg
     2041 aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca
     2101 acaacaattg cattcatttt atgtttcagg ttcaggggga ggtgtgggag gttttttaaa
     2161 gcaagtaaaa cctctacaaa tgtggtaaaa tcgataagga tccgtcgacc gatgcccttg
     2221 agagccttca acccagtcag ctccttccgg tgggcgcggg gcatgactat cgtcgccgca
     2281 cttatgactg tcttctttat catgcaactc gtaggacagg tgccggcagc gctcttccgc
     2341 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     2401 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     2461 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     2521 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     2581 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     2641 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     2701 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     2761 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     2821 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     2881 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     2941 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     3001 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     3061 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     3121 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     3181 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     3241 ctaaagtata tatgagtaaa cttggtctga cagcggccgc aaatgctaaa ccactgcagt
     3301 ggttaccagt gcttgatcag tgaggcaccg atctcagcga tctgcctatt tcgttcgtcc
     3361 atagtggcct gactccccgt cgtgtagatc actacgattc gtgagggctt accatcaggc
     3421 cccagcgcag caatgatgcc gcgagagccg cgttcaccgg cccccgattt gtcagcaatg
     3481 aaccagccag cagggagggc cgagcgaaga agtggtcctg ctactttgtc cgcctccatc
     3541 cagtctatga gctgctgtcg tgatgctaga gtaagaagtt cgccagtgag tagtttccga
     3601 agagttgtgg ccattgctac tggcatcgtg gtatcacgct cgtcgttcgg tatggcttcg
     3661 ttcaactctg gttcccagcg gtcaagccgg gtcacatgat cacccatatt atgaagaaat
     3721 gcagtcagct ccttagggcc tccgatcgtt gtcagaagta agttggccgc ggtgttgtcg
     3781 ctcatggtaa tggcagcact acacaattct cttaccgtca tgccatccgt aagatgcttt
     3841 tccgtgaccg gcgagtactc aaccaagtcg ttttgtgagt agtgtatacg gcgaccaagc
     3901 tgctcttgcc cggcgtctat acgggacaac accgcgccac atagcagtac tttgaaagtg
     3961 ctcatcatcg ggaatcgttc ttcggggcgg aaagactcaa ggatcttgcc gctattgaga
     4021 tccagttcga tatagcccac tcttgcaccc agttgatctt cagcatcttt tactttcacc
     4081 agcgtttcgg ggtgtgcaaa aacaggcaag caaaatgccg caaagaaggg aatgagtgcg
     4141 acacgaaaat gttggatgct catactcgtc ctttttcaat attattgaag catttatcag
     4201 ggttactagt acgtctctca aggataagta agtaatatta aggtacggga ggtattggac
     4261 aggccgcaat aaaatatctt tattttcatt acatctgtgt gttggttttt tgtgtgaatc
     4321 gatagtacta acatacgctc tccatcaaaa caaaacgaaa caaaacaaac tagcaaaata
     4381 ggctgtcccc agtgcaagtg caggtgccag aacatttctc t
//
