LOCUS       sequence-117238-         830 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  EGFP-based homologous recombination-dependent reporter for genome 
            editing with TALENs or CRISPR/Cas9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Flemr M, Buhler M
  TITLE     Single-Step Generation of Conditional Knockout Mouse Embryonic Stem 
            Cells.
  JOURNAL   Cell Rep. 2015 Jul 28;12(4):709-16. doi: 
            10.1016/j.celrep.2015.06.051. Epub 2015 Jul 16.
  PUBMED    26190102
REFERENCE   2  (bases 1 to 830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2015.06.051"; journalName: "Cell Rep"; date: 
            "2015-07-28- 28"; volume: "12"; issue: "4"; pages: "709-16"
COMMENT     Sequence Label: pMB1609_pRR-EGFP
FEATURES             Location/Qualifiers
     source          1..830
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        8..26
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      42..51
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(272..274,275..787)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIE"
     primer_bind     complement(575..594)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 gagtacttaa tacgactcac tataggctag cgctaccggt cgccaccatg gtgagcaagg
       61 gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg
      121 gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc
      181 tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc
      241 tgacctacta atgagagctc ttgacgtcta agtgagcaag ggcgaggagc tgttcaccgg
      301 ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc
      361 cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac
      421 cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg
      481 cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga
      541 aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc
      601 cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt
      661 caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt
      721 ctatatcatg gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa
      781 catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc
//
