LOCUS       sequence-117250-         929 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Constitutively active NFAT1 and also unable to interact with AP-1 
            with four point mutations in the DNA binding loop that abolish DNA 
            binding.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 929)
  AUTHORS   Martinez GJ, Pereira RM, Aijo T, Kim EY, Marangoni F, Pipkin ME, 
            Togher S, Heissmeyer V, Zhang YC, Crotty S, Lamperti ED, Ansel KM, 
            Mempel TR, Lahdesmaki H, Hogan PG, Rao A
  TITLE     The Transcription Factor NFAT Promotes Exhaustion of Activated CD8 T
            Cells.
  JOURNAL   Immunity. 2015 Feb 6. pii: S1074-7613(15)00032-1. doi: 
            10.1016/j.immuni.2015.01.006.
  PUBMED    25680272
REFERENCE   2  (bases 1 to 929)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Immunity. 
            2015 Feb 6. pii: S1074-7613(15)00032-1. doi: 
            10.1016/j.immuni.2015.01.006."
COMMENT     Sequence Label: DBDmut-CA-RIT-NFAT1
FEATURES             Location/Qualifiers
     source          1..929
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    10..34
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             79..168
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
ORIGIN
        1 gaggtcatca caagtttgta caaaaaagca ggcttcgaag gagatagaac caattcttaa
       61 gcttccacca tgatctttta cccatacgat gttcctgact atgcgggcta tccctatgac
      121 gtcccggact atgcaggatc ctatccatat gacgttccag attacgctgc tcagtgcggc
      181 cgcgacgtcc cggagccgca gcccgacccc gatggcgggg acggccccgg ccacgagccc
      241 gggggcagtc cccaagacga gctggacttt tccatcctct tcgattatga ctatctgaac
      301 cctatcgaag aagaaccgat cgcacataag gccatcagct caccctccgg actcgcatac
      361 ccggatgatg tcctggacta tggcctcaag ccatgcaacc cccttgccag tctctctggc
      421 gagccccctg gccggttcgg agagccggat agtatagggt tccagaactt tctgagcccg
      481 gtcaagccag caggggcttc gggcccgagc cctcggatcg agatcactcc atcccacgaa
      541 ctgatgcagg cagggggggc cctccgtggg agagacgccg gcctgtcccc cgagcagccg
      601 gccctggccc tggccggcgt ggccgccagc ccgaggttca cactgcccgt gcccggctac
      661 gagggctacc gcgagccgct ttgcttgagc cccgctgcag caggcgcagc agccgcattc
      721 atttctgaca ccttctcccc ctacacctcg ccctgcgtct cacccaataa cgccgggccc
      781 gacgacctgt gtccccagtt tcaaaacatc cctgctcatt atgccccccg gaccgctcca
      841 ataatggcac ctcgaaccag cctcgccgag gacagctgcc tgggccgaca ctcgcccgtg
      901 ccccgtccgg catcccgctc ctcctcacc
//
