LOCUS       sequence-117253-         704 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  co-expression of Cas9 and a sgRNA targeting K08F4.2 in the middle of
            the ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 704)
  AUTHORS   Paix A, Wang Y, Smith HE, Lee CY, Calidas D, Lu T, Smith J, Schmidt 
            H, Krause MW, Seydoux G
  TITLE     Scalable and versatile genome editing using linear DNAs with 
            microhomology to Cas9 Sites in Caenorhabditis elegans.
  JOURNAL   Genetics. 2014 Dec;198(4):1347-56. doi: 10.1534/genetics.114.170423.
            Epub 2014 Sep 23.
  PUBMED    25249454
REFERENCE   2  (bases 1 to 704)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.114.170423"; journalName: "Genetics"; date: 
            "2014-12"; volume: "198"; issue: "4"; pages: "1347-56"
COMMENT     Sequence Label: APq5271
FEATURES             Location/Qualifiers
     source          1..704
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        281..356
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     primer_bind     628..650
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 ctgctgagac ctgaaaatag caactttagt actactataa tttgtcaacc ttttcaaaaa
       61 aagcatgcaa tttttgagaa actcttataa aagctattat taaaaaaaca ccttttttcc
      121 aaaattattc cacaaaaaat atgttatgaa atgcctacac cctctcacac acactcttta
      181 tactactctg tcaaactcac gagatgtctg ccgcctcttg tgttgcccct atataaacac
      241 ctcctattgc gagatgtctt gaatcactcc ctgtttgtgt gttttagagc tagaaatagc
      301 aagttaaaat aaggctagtc cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt
      361 tgtgaaattt ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc
      421 gcagcctgaa tggcgaatgg cgcctgatgc ggtattttct ccttacgcat ctgtgcggta
      481 tttcacaccg catatggtgc actctcagtg caatctgctc tgatgccgca tagttaagcc
      541 agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat
      601 ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt
      661 catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg ccta
//
