LOCUS       sequence-117256-         739 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  co-expression of Cas9 and a sgRNA targeting K08F4.2 in the middle of
            the ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 739)
  AUTHORS   Paix A, Wang Y, Smith HE, Lee CY, Calidas D, Lu T, Smith J, Schmidt 
            H, Krause MW, Seydoux G
  TITLE     Scalable and versatile genome editing using linear DNAs with 
            microhomology to Cas9 Sites in Caenorhabditis elegans.
  JOURNAL   Genetics. 2014 Dec;198(4):1347-56. doi: 10.1534/genetics.114.170423.
            Epub 2014 Sep 23.
  PUBMED    25249454
REFERENCE   2  (bases 1 to 739)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.114.170423"; journalName: "Genetics"; date: 
            "2014-12"; volume: "198"; issue: "4"; pages: "1347-56"
COMMENT     Sequence Label: APq5271
FEATURES             Location/Qualifiers
     source          1..739
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(8..331)
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     CDS             complement(346..372)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(373..393)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 gttgtgagac ttttttcttg gcggcacaat aaagtttcct ctttttgttg ggtcactcaa
       61 cgaacacaat acaaatattt gagcgctcgc atttttctca acctttcggg tttaaagcac
      121 ttcaaaatgg aattgaagag agattgtgaa agtacatcag acagaatcac attttgatag
      181 cattcacttc actcagatgc aaacttttca gtgactataa aaagtcataa aataataaca
      241 ttaaaaaagc aaataaatta gcgagagaat tttttgacaa aaagaaagaa gagtgataga
      301 gaagaaggga atgcttgaaa ggattttgca tttatcacta gtttaggcgt agtctgggac
      361 gtcgtatggg tagaccttac gcttcttctt tggtggtccg gatcctccgt ctcctccgag
      421 ttgggagagg tcgatacggg tctcgtagag tccggtgatg gattggtgga tgagggtggc
      481 gtcgaggacc tccttggtgg aggtgtaacg cttacggtcg atggtggtgt cgaagtactt
      541 gaaggcggct ggggctccga ggttggtgag ggtgaagagg tggatgatgt tctcggcttg
      601 ctcacggatt ggcttgtcac ggtgcttgtt gtaggcggag aggaccttgt cgaggttggc
      661 gtcggcgagg atgacacgct tggagaactc ggagatttgc tcgatgatct cgtcgaggta
      721 gtgcttgtgt tgctcgacg
//
