LOCUS       sequence-117257-         506 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  co-expression of Cas9 and a sgRNA targeting K08F4.2 in the middle of
            the ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 506)
  AUTHORS   Paix A, Wang Y, Smith HE, Lee CY, Calidas D, Lu T, Smith J, Schmidt 
            H, Krause MW, Seydoux G
  TITLE     Scalable and versatile genome editing using linear DNAs with 
            microhomology to Cas9 Sites in Caenorhabditis elegans.
  JOURNAL   Genetics. 2014 Dec;198(4):1347-56. doi: 10.1534/genetics.114.170423.
            Epub 2014 Sep 23.
  PUBMED    25249454
REFERENCE   2  (bases 1 to 506)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.114.170423"; journalName: "Genetics"; date: 
            "2014-12"; volume: "198"; issue: "4"; pages: "1347-56"
COMMENT     Sequence Label: APq5210
FEATURES             Location/Qualifiers
     source          1..506
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        complement(290..365)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac
       61 aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg
      121 catcagagca gattgcactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg
      181 taaggagaaa ataccgcatc aggcgccatt cgccattcag gctgcgcaac tgttgggaag
      241 ggcgatcggt gcgggcctct tcgctattac gccagaaatt tcacaaaaag caccgactcg
      301 gtgccacttt ttcaagttga taacggacta gccttatttt aacttgctat ttctagctct
      361 aaaacacaca aacagggagt gattcaagac atctcgcaat aggaggtgtt tatatagggg
      421 caacacaaga ggcggcagac atctcgtgag tttgacagag tagtataaag agtgtgtgtg
      481 agagggtgta ggcatttcat aacata
//
