LOCUS       sequence-117260-         502 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  co-expression of Cas9 and a sgRNA targeting 5'end of C.elegans 
            K08F4.2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 502)
  AUTHORS   Paix A, Wang Y, Smith HE, Lee CY, Calidas D, Lu T, Smith J, Schmidt 
            H, Krause MW, Seydoux G
  TITLE     Scalable and versatile genome editing using linear DNAs with 
            microhomology to Cas9 Sites in Caenorhabditis elegans.
  JOURNAL   Genetics. 2014 Dec;198(4):1347-56. doi: 10.1534/genetics.114.170423.
            Epub 2014 Sep 23.
  PUBMED    25249454
REFERENCE   2  (bases 1 to 502)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.114.170423"; journalName: "Genetics"; date: 
            "2014-12"; volume: "198"; issue: "4"; pages: "1347-56"
COMMENT     Sequence Label: AP303-3
FEATURES             Location/Qualifiers
     source          1..502
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        complement(286..361)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc
       61 ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc
      121 agagcagatt gcactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag
      181 gagaaaatac cgcatcaggc gccattcgcc attcaggctg cgcaactgtt gggaagggcg
      241 atcggtgcgg gcctcttcgc tattacgcca gaaatttcac aaaaagcacc gactcggtgc
      301 cactttttca agttgataac ggactagcct tattttaact tgctatttct agctctaaaa
      361 ctcttattct gggttaaggc caagacatct cgcaatagga ggtgtttata taggggcaac
      421 acaagaggcg gcagacatct cgtgagtttg acagagtagt ataaagagtg tgtgtgagag
      481 ggtgtaggca tttcataaca ta
//
