LOCUS       sequence-117263-         739 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Luciferase assay vector, assessing regulation of 85 nucleotide CA 
            repeats.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 739)
  TITLE     RNA-binding protein 3' UTR target plasmids (unpublished)
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 739)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: psicheck_85_CA_repeat
FEATURES             Location/Qualifiers
     source          1..739
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     intron          133..265
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     248..267
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        310..328
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      332..341
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc
       61 aaaaagcttg attcttctga cacaacagtc tcgaacttaa gctgcagaag ttggtcgtga
      121 ggcactgggc aggtaagtat caaggttaca agacaggttt aaggagacca atagaaactg
      181 ggcttgtcga gacagagaag actcttgcgt ttctgatagg cacctattgg tcttactgac
      241 atccactttg cctttctctc cacaggtgtc cactcccagt tcaattacag ctcttaaggc
      301 tagagtactt aatacgactc actataggct agccaccatg gcttccaagg tgtacgaccc
      361 cgagcaacgc aaacgcatga tcactgggcc tcagtggtgg gctcgctgca agcaaatgaa
      421 cgtgctggac tccttcatca actactatga ttccgagaag cacgccgaga acgccgtgat
      481 ttttctgcat ggtaacgctg cctccagcta cctgtggagg cacgtcgtgc ctcacatcga
      541 gcccgtggct agatgcatca tccctgatct gatcggaatg ggtaagtccg gcaagagcgg
      601 gaatggctca tatcgcctcc tggatcacta caagtacctc accgcttggt tcgagctgct
      661 gaaccttcca aagaaaatca tctttgtggg ccacgactgg ggggcttgtc tggcctttca
      721 ctactcctac gagcaccaa
//
