LOCUS       sequence-117267-         742 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  ePathBrick pETM6 vector encoding CsF3H, CsDFR, and DuLAR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 742)
  AUTHORS   Zhao S, Jones JA, Lachance DM, Bhan N, Khalidi O, Venkataraman S, 
            Wang Z, Koffas MA
  TITLE     Improvement of catechin production in Escherichia coli through 
            combinatorial metabolic engineering.
  JOURNAL   Metab Eng. 2014 Dec 17;28C:43-53. doi: 10.1016/j.ymben.2014.12.002.
  PUBMED    25527438
REFERENCE   2  (bases 1 to 742)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab 
            Eng."; date: "2014-12-17"; volume: "28C:43-53. doi"; pages: " 
            10.1016/j.ymben.2014.12.002"
COMMENT     Sequence Label: p158
FEATURES             Location/Qualifiers
     source          1..742
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     44..63
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        44..62
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    63..87
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             102..124
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             279..290
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 ccggcgtagc ctaggatcga gatcgatctc gatcccgcga aattaatacg actcactata
       61 ggggaattgt gagcggataa caattcccct ctagaaataa ttttgtttaa ctttaagaag
      121 gagatataca tatggcaccg accaccaccc tgaccgcact ggcagaagaa aaaagcctgc
      181 agcagaaatt tgttcgtgat gaagatgaac gtccgaaagt tgcctataat gtgtttagca
      241 atgaaatccc ggttattagc ctggcaggta ttgatgaaat tgaaggtcgt cgtagcgaaa
      301 tttgccgtaa aattgttgaa gcatgtgaag gttggggtgt ttttcaggtt gttgatcatg
      361 gtgttgatgc aaatctgatt gcagaaatga cccgtctggc acgtgaattt tttgcactgc
      421 ctccggaaga aaaactgcgt tttgatatga gcggtggtaa aaaaggtggt tttattgtta
      481 gcagccatct gcagggtgaa gcagttcagg attggcgtga aattgttacc tatttcagct
      541 atccgattcg tgcacgtgat tatagccgtt ggcctgataa accggaaggt tggcgtgcag
      601 ttaccgaaac ctatagcgaa aaactgatgg atctggcatg taaactgctg gaagttctga
      661 gcgaagcaat gggtctggaa aaagaggcac tgaccaaagc atgtgttgat atggatcaga
      721 aagtggtgat caacttctat cc
//
