LOCUS       sequence-117268-         820 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses two dimeric Broccoli aptamers in the F30 scaffold in 
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 820)
  AUTHORS   Filonov GS, Kam CW, Song W, Jaffrey SR
  TITLE     In-gel imaging of RNA processing using broccoli reveals optimal 
            aptamer expression strategies.
  JOURNAL   Chem Biol. 2015 May 21;22(5):649-60. doi: 
            10.1016/j.chembiol.2015.04.018.
  PUBMED    26000751
REFERENCE   2  (bases 1 to 820)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.chembiol.2015.04"; journalName: "Chem Biol"; date: 
            "2015-05-21- 21"; volume: "22"; issue: "5"; pages: "649-60"
COMMENT     Sequence Label: pAVU6+27-F30-2xdBroccoli
FEATURES             Location/Qualifiers
     source          1..820
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     169..188
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     polyA_signal    624..745
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(661..680)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     715..734
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 ggcctatttc ccatgattcc ttcatatttg catatacgat acaaggctgt tagagagata
       61 attagaatta atttgactgt aaacacaaag atattagtac aaaatacgtg acgtagaaag
      121 taataatttc ttgggtagtt tgcagtttta aaattatgtt ttaaaatgga ctatcatatg
      181 cttaccgtaa cttgaaagta tttcgatttc ttggctttat atatcttgtg gaaaggacga
      241 aacaccgtgc tcgcttcggc agcacatata ctagtcgact tgccatgtgt atgtgggaga
      301 cggtcgggtc catctgagac ggtcgggtcc agatattcgt atctgtcgag tagagtgtgg
      361 gctcagatgt cgagtagagt gtgggctccc acatactctg atgatccaga cggtcgggtc
      421 catctgagac ggtcgggtcc agatattcgt atctgtcgag tagagtgtgg gctcagatgt
      481 cgagtagagt gtgggctgga tcattcatgg caatctagag cggacttcgg tccgcttttt
      541 actaggacct gcaggcatgc aagcttgacg tcggttaccg atatccatat ggcggccgca
      601 tcgatctcga gccgcggact agtaacttgt ttattgcagc ttataatggt tacaaataaa
      661 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
      721 tgtccaaact catcaatgta tcttatcatg tcttacgtag ataagtagca tggcgggtta
      781 atcattaact acaaggaacc cctagtgatg gagttggcca
//
