LOCUS       sequence-117270-         533 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes accA, accB, accC, accD in monocistronic configuration..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 533)
  AUTHORS   Xu P, Gu Q, Wang W, Wong L, Bower AG, Collins CH, Koffas MA
  TITLE     Modular optimization of multi-gene pathways for fatty acids 
            production in E. coli.
  JOURNAL   Nat Commun. 2013;4:1409. doi: 10.1038/ncomms2425.
  PUBMED    23361000
REFERENCE   2  (bases 1 to 533)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2013"; volume: "4:1409. doi"; pages: " 
            10.1038/ncomms2425"
COMMENT     Sequence Label: pETM6-M.accABCD
FEATURES             Location/Qualifiers
     source          1..533
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     5..24
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        5..23
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    24..48
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             63..85
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 aaattaatac gactcactat aggggaattg tgagcggata acaattcccc tctagaaata
       61 attttgttta actttaagaa ggagatatac ataatacata tgagtctgaa tttccttgat
      121 tttgaacagc cgattgcana gctggaagcg aaaatcgatt ctctgactgc ggttagccgt
      181 caggatgaga aactggatat taacatcgat gaagaagtgc atcgtctgcg tgaaaaaagc
      241 gtagaactga cacgtaaaat cttcgccgat ctcggtgcat ggcagattgc gcaactggca
      301 cgccatccac agcgtcctta taccctggat tacgttcgcc tggcatttga tgaatttgac
      361 gaactggctg gcgaccgcgc gtatgcagac gataaagcta tcgtcggtgg tatcgcccgt
      421 ctcgatggtc gtccggtgat gatcattggt catcaaaaag gtcgtgaaac caaagaaaaa
      481 attcgccgta actttggtat gccancgcca gaaggttacc gcaaagcact gcg
//
