LOCUS       sequence-117273-         819 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  ePathBrick pETM6 vector encoding CsF3H, FaDFR, and DuLAR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 819)
  AUTHORS   Zhao S, Jones JA, Lachance DM, Bhan N, Khalidi O, Venkataraman S, 
            Wang Z, Koffas MA
  TITLE     Improvement of catechin production in Escherichia coli through 
            combinatorial metabolic engineering.
  JOURNAL   Metab Eng. 2014 Dec 17;28C:43-53. doi: 10.1016/j.ymben.2014.12.002.
  PUBMED    25527438
REFERENCE   2  (bases 1 to 819)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab 
            Eng."; date: "2014-12-17"; volume: "28C:43-53. doi"; pages: " 
            10.1016/j.ymben.2014.12.002"
COMMENT     Sequence Label: p168
FEATURES             Location/Qualifiers
     source          1..819
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     52..71
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        52..70
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    71..95
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             110..132
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             287..298
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 cgatgcgtcc ggcgtagcct aggatcgaga tcgatctcga tcccgcgaaa ttaatacgac
       61 tcactatagg ggaattgtga gcggataaca attcccctct agaaataatt ttgtttaact
      121 ttaagaagga gatatacata tggcaccgac caccaccctg accgcactgg cagaagaaaa
      181 aagcctgcag cagaaatttg ttcgtgatga agatgaacgt ccgaaagttg cctataatgt
      241 gtttagcaat gaaatcccgg ttattagcct ggcaggtatt gatgaaattg aaggtcgtcg
      301 tagcgaaatt tgccgtaaaa ttgttgaagc atgtgaaggt tggggtgttt ttcaggttgt
      361 tgatcatggt gttgatgcaa atctgattgc agaaatgacc cgtctggcac gtgaattttt
      421 tgcactgcct ccggaagaaa aactgcgttt tgatatgagc ggtggtaaaa aaggtggttt
      481 tattgttagc agccatctgc agggtgaagc agttcaggat tggcgtgaaa ttgttaccta
      541 tttcagctat ccgattcgtg cacgtgatta tagccgttgg cctgataaac cggaaggttg
      601 gcgtgcagtt accgaaacct atagcgaaaa actgatggat ctggcatgta aactgctgga
      661 agttctgagc gaagcaatgg gtctggaaaa agaggcactg accaaagcat gtgttgatat
      721 ggatcagaaa gtggtgatca acttctatcc gaaatgtccg cagccggatc tgaccctggg
      781 tctgaaacgt cataccgatc cgggtacaat taccctgct
//
