LOCUS       sequence-117275-         879 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses mCherry under the control of the Concensus T7 Promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 879)
  AUTHORS   Jones JA, Vernacchio VR, Lachance DM, Lebovich M, Fu L, Shirke AN, 
            Schultz VL, Cress B, Linhardt RJ, Koffas MA
  TITLE     ePathOptimize: A Combinatorial Approach for Transcriptional 
            Balancing of Metabolic Pathways.
  JOURNAL   Sci Rep. 2015 Jun 11;5:11301. doi: 10.1038/srep11301.
  PUBMED    26062452
REFERENCE   2  (bases 1 to 879)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2015-06-11"; volume: "5:11301. doi"; pages: " 
            10.1038/srep11301"
COMMENT     Sequence Label: pETM6-mCherry
FEATURES             Location/Qualifiers
     source          1..879
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     52..71
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        52..70
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    71..95
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             110..132
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 cgatgcgtcc ggcgtagcct aggatcgaga tcgatctcga tcccgcgaaa ttaatacgac
       61 tcactatagg ggaattgtga gcggataaca attcccctct agaaataatt ttgtttaact
      121 ttaagaagga gatatacata tggtttcaaa aggcgaagaa gacaacatgg cgattatcaa
      181 ggaatttatg cgtttcaagg tccacatgga aggcagcgtc aatggtcacg aatttgaaat
      241 tgaaggcgaa ggtgaaggcc gtccgtatga aggcacccag acggcaaaac tgaaggtcac
      301 caaaggcggt ccgctgccgt ttgcttggga tattctgtca ccgcaattca tgtatggttc
      361 gaaagcgtac gttaagcatc cggccgatat cccggactat ctgaaactgt cctttccgga
      421 aggcttcaaa tgggaacgtg ttatgaactt cgaagatggc ggtgtggtta ccgtcacgca
      481 ggatagctct ctgcaagacg gtgaatttat ttataaagtg aagctgcgcg gcaccaattt
      541 cccgagcgat ggtccggtta tgcagaaaaa gacgatgggc tgggaagcga gttccgaacg
      601 tatgtacccg gaagacggtg ccctgaaagg cgaaatcaag cagcgcctga aactgaagga
      661 tggcggtcac tatgacgcag aagtgaaaac cacgtacaag gctaaaaagc cggtccaact
      721 gccgggtgca tacaacgtga acatcaagct ggatatcacc agccataacg aagactatac
      781 gatcgttgaa cagtacgaac gtgcagaagg ccgccactct accggcggta tggatgaact
      841 gtacaaataa ggtaccctcg agtctggtaa agaaaccgc
//
