LOCUS       sequence-117278-         951 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Second generation Rac1 FRET biosensor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 951)
  AUTHORS   Fritz RD, Menshykau D, Martin K, Reimann A, Pontelli V, Pertz O
  TITLE     SrGAP2-Dependent Integration of Membrane Geometry and 
            Slit-Robo-Repulsive Cues Regulates Fibroblast Contact Inhibition of 
            Locomotion.
  JOURNAL   Dev Cell. 2015 Sep 29. pii: S1534-5807(15)00582-1. doi: 
            10.1016/j.devcel.2015.09.002.
  PUBMED    26439400
REFERENCE   2  (bases 1 to 951)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Cell. 
            2015 Sep 29. pii: S1534-5807(15)00582-1. doi: 
            10.1016/j.devcel.2015.09.002."
COMMENT     Sequence Label: pTriEx4-Rac1-2G
FEATURES             Location/Qualifiers
     source          1..951
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    18..42
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        58..167
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     CDS             188..205
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             215..244
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 atacgactca ctatagggga attgtgagcg gataacaatt ccccggagtt aatccgggac
       61 ctttaattca acccaacaca atatattata gttaaataag aattattatc aaatcatttg
      121 tatattaatt aaaatactat actgtaaatt acattttatt tacaatcaaa ggagatatac
      181 catggcacac catcaccacc atcacgggag cggggagcag aaactaatat cagaggagga
      241 tcttggctca ggatcctcca ccgacggcat ggacgagctg tacaagggtg ctagcggagg
      301 aatggtgagc aagggcgagg agaccacaat gggcgtaatc aagcccgaca tgaagatcaa
      361 gctgaagatg gagggcaacg tgaatggcca cgccttcgtg atcgagggcg agggcgaggg
      421 caagccctac gacggcacca acaccatcaa cctggaggtg aaggagggag cccccctgcc
      481 cttctcctac gacattctga ccaccgcgtt cgcctacggc aacagggcct tcaccaagta
      541 ccccgacgac atccccaact acttcaagca gtccttcccc gagggctact cttgggagcg
      601 caccatgacc ttcgaggaca agggcatcgt gaaggtgaag tccgacatct ccatggagga
      661 ggactccttc atctacgaga tacacctcaa gggcgagaac ttccccccca acggccccgt
      721 gatgcagaag aagaccaccg gctgggacgc ctccaccgag aggatgtacg tgcgcgacgg
      781 cgtgctgaag ggcgacgtca agcacaagct gctgctggag ggcggcggcc accaccgcgt
      841 tgacttcaag accatctaca gggccaagaa ggcggtgaag ctgcccgact atcactttgt
      901 ggaccaccgc atcgagatcc tgaaccacga caaggactac aacaaggtga c
//
