LOCUS       sequence-117280-         908 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses 5S rRNA fused to two dimeric Broccoli aptamers in the F30 
            scaffold in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 908)
  AUTHORS   Filonov GS, Jaffrey SR
  TITLE     RNA Imaging with Dimeric Broccoli in Live Bacterial and Mammalian 
            Cells.
  JOURNAL   Curr Protoc Chem Biol. 2016 Mar 16;8(1):1-28. doi: 
            10.1002/9780470559277.ch150174.
  PUBMED    26995352
REFERENCE   2  (bases 1 to 908)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1002/9780470559277"; journalName: "Curr Protoc Chem Biol"; date:
            "2016-03-16- 16"; volume: "8"; issue: "1"; pages: "1-28"
COMMENT     Sequence Label: pAV5S-F30-2xdBroccoli
FEATURES             Location/Qualifiers
     source          1..908
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(543..562)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
ORIGIN
        1 ttttatattt accttagagc tttaaatctc tgtaggtagt ttgtccaatt atgtcacacc
       61 acagaagtaa ggttccttca caaagatccg gaccaaagcg gccatcgtgc ctccccactc
      121 ctgcagttcg ggggcatgga tgcgcggata gccgctgctg gtttcctgga tgccgacgga
      181 tttgcactgc cggtagaact ccgcgaggtc gtccagcctc aggcagcagc tgaaccaact
      241 cgcgagggga tcgagcccgg ggtgggcgaa gaactccagc atgagatccc cgcgctggag
      301 gatcatccag ccggcgtccc ggaaaacgat tccgaagccc aacctttcat agaaggcggc
      361 ggtggaatcg aaatctcgtg atggcaggtt gggcgtcgct tggtcggtca tttcgaaccc
      421 cagagtcccg ctcagaagaa ctcgtcaaga aggcgataga aggcgatgcg ctgcgaatcg
      481 ggagcggcga taccgtaaag cacgaggaag cggtcagccc attcgccgcc aagctcttca
      541 gcaatatcac gggtagccaa cgctatgtcc tgatagcggt ccgccacacc cagccggcca
      601 cagtcgatga atccagaaaa gcggccattt tccaccatga tattcggcaa gcaggcatcg
      661 ccatgggtca cgacgagatc ctcgccgtcg ggcatgcgcg ccttgagcct ggcgaacagt
      721 tcggctggcg cgagcccctg atgctcttcg tccagatcat cctgatcgac aagaccggct
      781 tccatccgag tacgtgctcg ctcgatgcga tgtttcgctt ggtggtcgaa tgggcaggta
      841 gccggatcaa gcgtatgcag ccgccgcatt gcatcagcca tgatggatac tttctcggca
      901 ggagcaag
//
