LOCUS       sequence-117283-         749 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses 5S rRNA fused to two dimeric Broccoli aptamers in the F30 
            scaffold in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 749)
  AUTHORS   Filonov GS, Jaffrey SR
  TITLE     RNA Imaging with Dimeric Broccoli in Live Bacterial and Mammalian 
            Cells.
  JOURNAL   Curr Protoc Chem Biol. 2016 Mar 16;8(1):1-28. doi: 
            10.1002/9780470559277.ch150174.
  PUBMED    26995352
REFERENCE   2  (bases 1 to 749)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1002/9780470559277"; journalName: "Curr Protoc Chem Biol"; date:
            "2016-03-16- 16"; volume: "8"; issue: "1"; pages: "1-28"
COMMENT     Sequence Label: pAV5S-F30-2xdBroccoli
FEATURES             Location/Qualifiers
     source          1..749
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    538..659
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(575..594)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     629..648
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tcggatgcaa tccggcgggc acggccgggc cgggctgggc tcttggggca gccaggcgcc
       61 tccttcagcg tctacggcca taccaccctg aacgcgcccg atctcgtctg atctcggaag
      121 ctaagcaggg tcgggcctgg ttagtacttg gatgggagac cgcctgggaa taccgggtgc
      181 tgtaggcgtc gacttgccat gtgtatgtgg gagacggtcg ggtccatctg agacggtcgg
      241 gtccagatat tcgtatctgt cgagtagagt gtgggctcag atgtcgagta gagtgtgggc
      301 tcccacatac tctgatgatc cagacggtcg ggtccatctg agacggtcgg gtccagatat
      361 tcgtatctgt cgagtagagt gtgggctcag atgtcgagta gagtgtgggc tggatcattc
      421 atggcaatct agagcggact tcggtccgct ttttactagg acctgcaggc atgcaagctt
      481 gacgtcggtt accgatatcc atatggcggc cgcatcgatc tcgagccgcg gactagtaac
      541 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
      601 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
      661 catgtcttac gtagataagt agcatggcgg gttaatcatt aactacaagg aacccctagt
      721 gatggagttg gccactccct ctctgcgcg
//
