LOCUS       sequence-117284-         741 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Second generation Rac1 FRET biosensor for adenovirus production.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 741)
  AUTHORS   Fritz RD, Menshykau D, Martin K, Reimann A, Pontelli V, Pertz O
  TITLE     SrGAP2-Dependent Integration of Membrane Geometry and 
            Slit-Robo-Repulsive Cues Regulates Fibroblast Contact Inhibition of 
            Locomotion.
  JOURNAL   Dev Cell. 2015 Sep 29. pii: S1534-5807(15)00582-1. doi: 
            10.1016/j.devcel.2015.09.002.
  PUBMED    26439400
REFERENCE   2  (bases 1 to 741)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Cell. 
            2015 Sep 29. pii: S1534-5807(15)00582-1. doi: 
            10.1016/j.devcel.2015.09.002."
COMMENT     Sequence Label: pAD/CMV/V5-DEST-Rac1-2G
FEATURES             Location/Qualifiers
     source          1..741
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        47..65
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    95..119
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        201..219
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    220..244
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        261..368
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     CDS             389..406
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             416..445
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 ctctctggct aactagagaa cccactgctt actggcttat cgaaattaat acgactcact
       61 atagggagac ccaagctggc tagttaagct atcaacaagt ttgtacaaaa aagcaggctt
      121 taaaggaacc aattcagtcg actggatccg gttattgtgc tgtctcatca ttttggcaaa
      181 gaattggatc ggaccgaaat taatacgact cactataggg gaattgtgag cggataacaa
      241 ttccccggag cttaatccgg gacctttaat tcaacccaac acaatatatt atagttaaat
      301 aagaattatt atcaaatcat ttgtatatta taaaatacta tactgtaaat tacattttat
      361 ttacaatcaa aggagatata ccatggcaca ccatcaccac catcacggna gcggggagca
      421 gaaactaata tcagaggagg atcttggctc aggatcctcc accgacggca tggacgagct
      481 gtacaagggt gctagcggag gaatggtgag caagggcgag gagaccacaa tgggcgtaat
      541 caagcccgac atgaanatca agctgaagat ggagggcaac gtgaatggcc acgccttcgt
      601 gatcgagggc gagggcgagg gcaagcccta cgacggcacc aacaccatca acctggaggt
      661 gaaggaggga gcccccctgc ccttctccta cgacattctg accaccgcgt tcgcctacgg
      721 caacagggcc ttcaccaagt a
//
