LOCUS       sequence-117285-         899 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Broccoli in the F30 scaffold in bacterial cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 899)
  AUTHORS   Filonov GS, Kam CW, Song W, Jaffrey SR
  TITLE     In-gel imaging of RNA processing using broccoli reveals optimal 
            aptamer expression strategies.
  JOURNAL   Chem Biol. 2015 May 21;22(5):649-60. doi: 
            10.1016/j.chembiol.2015.04.018.
  PUBMED    26000751
REFERENCE   2  (bases 1 to 899)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.chembiol.2015.04"; journalName: "Chem Biol"; date: 
            "2015-05-21- 21"; volume: "22"; issue: "5"; pages: "649-60"
COMMENT     Sequence Label: pET28c-F30-Broccoli
FEATURES             Location/Qualifiers
     source          1..899
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 ccggtgccta atgagtgagc taacttacat taattgcgtt gcgctcactg cccgctttcc
       61 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
      121 gtttgcgtat tgggcgccag ggtggttttt cttttcacca gtgagacggg caacagctga
      181 ttgcccttca ccgcctggcc ctgagagagt tgcagcaagc ggtccacgct ggtttgcccc
      241 agcaggcgaa aatcctgttt gatggtggtt aacggcggga tataacatga gctgtcttcg
      301 gtatcgtcgt atcccactac cgagatatcc gcaccaacgc gcagcccgga ctcggtaatg
      361 gcgcgcattg cgcccagcgc catctgatcg ttggcaacca gcatcgcagt gggaacgatg
      421 ccctcattca gcatttgcat ggtttgttga aaaccggaca tggcactcca gtcgccttcc
      481 cgttccgcta tcggctgaat ttgattgcga gtgagatatt tatgccagcc agccagacgc
      541 agacgcgccg agacagaact taatgggccc gctaacagcg cgatttgctg gtgacccaat
      601 gcgaccagat gctccacgcc cagtcgcgta ccgtcttcat gggagaaaat aatactgttg
      661 atgggtgtct ggtcagagac atcaagaaat aacgccggaa cattagtgca ggcagcttcc
      721 acagcaatgg catcctggtc atccagcgga tagttaatga tcagcccact gacgcgttgc
      781 gcgagaagat tgtgcaccgc cgctttacag gcttcgacgc cgcttcgttc taccatcgac
      841 accaccacgc tggcacccag ttgatcggcg cgagatttaa tcgccgcgac aattttgcg
//
