LOCUS       sequence-117288-         992 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Human cytoplasmic dynein heavy chain (DYNC1H1) codon optimized for 
            Sf9 expression with a SNAPf tag for fluorescent labeling and a 
            TEV-cleavable His-ZZ tag for purification 
            (His-ZZ-TCS-SNAPf-DYNC1H1)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 992)
  AUTHORS   Schlager MA, Hoang HT, Urnavicius L, Bullock SL, Carter AP
  TITLE     In vitro reconstitution of a highly processive recombinant human 
            dynein complex.
  JOURNAL   EMBO J. 2014 Sep 1;33(17):1855-68. doi: 10.15252/embj.201488792. 
            Epub 2014 Jul 1.
  PUBMED    24986880
REFERENCE   2  (bases 1 to 992)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.15252/embj.201488792"; journalName: "EMBO J"; date: "2014-09-1- 
            1"; volume: "33"; issue: "17"; pages: "1855-68"
COMMENT     Sequence Label: pDyn1
FEATURES             Location/Qualifiers
     source          1..992
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             97..120
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             568..588
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             610..630
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 ttttcgtaac agttttgtaa taaaaaaacc tataaatatt ccggattatt cataccgtcc
       61 caccatcggg cgcggatcct caacatgtct tactatcacc accaccatca ccaccatcac
      121 ggttcaatgt gcatggctgg cctcgcccag cacgacgaag ctgttgacaa taagttcaac
      181 aaggaacagc agaacgcttt ctacgaaatc ctccacctgc caaacctcaa cgaagaacag
      241 cgcaacgcat tcatccaatc actgaaggac gacccatccc aatccgcaaa cctgttggcc
      301 gaagcaaaga agctgaacga cgctcaggct cccaaagtgg acaataagtt caataaagag
      361 cagcagaacg ccttctatga gattctgcac ttgcctaacc tgaacgagga acagcgcaat
      421 gctttcatcc agtctctgaa ggatgatcca tcacagagcg ctaatttgct ggccgaggcc
      481 aagaagctca atgatgctca agctcccaag gtggatgcta actccgccgg aaagtccact
      541 gactacgaca tccctacaac tgcaagcgag aacctctact tccaaggcga ctatgacatc
      601 ccaactacag agaatctcta tttccagggt gacaaagact gcgaaatgaa gcgcaccacc
      661 ctggatagcc ctctgggcaa gctggaactg tctgggtgcg aacagggcct gcaccgtatc
      721 atcttcctgg gcaaaggaac atctgccgcc gacgccgtgg aagtgcctgc cccagccgcc
      781 gtgctgggcg gaccagagcc actgatgcag gccaccgcct ggctcaacgc ctactttcac
      841 cagcctgagg ccatcgagga gttccctgtg ccagccctgc accacccagt gttccagcag
      901 gagagcttta cccgccaggt gctgtggaaa ctgctgaaag tggtgaagtt cggagaggtc
      961 atcagctaca gccacctggc cgccctggcc gg
//
