LOCUS       sequence-117289-         761 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Broccoli in the F30 scaffold in bacterial cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 761)
  AUTHORS   Filonov GS, Kam CW, Song W, Jaffrey SR
  TITLE     In-gel imaging of RNA processing using broccoli reveals optimal 
            aptamer expression strategies.
  JOURNAL   Chem Biol. 2015 May 21;22(5):649-60. doi: 
            10.1016/j.chembiol.2015.04.018.
  PUBMED    26000751
REFERENCE   2  (bases 1 to 761)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.chembiol.2015.04"; journalName: "Chem Biol"; date: 
            "2015-05-21- 21"; volume: "22"; issue: "5"; pages: "649-60"
COMMENT     Sequence Label: pET28c-F30-Broccoli
FEATURES             Location/Qualifiers
     source          1..761
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(10..57)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     53..71
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     CDS             complement(124..141)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      complement(148..195)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(302..321)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(303..321)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(405..424)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        630..707
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     complement(727..746)
                     /label=LacI-R
                     /note="LacI, reverse primer"
ORIGIN
        1 tcctttcagc aaaaaacccc tcaagacccg tttagaggcc ccaaggggtt atgctagtta
       61 ttgctcagcg gtggcagcag ccaactcagc ttcctttcgg gctttgttag cagccggatc
      121 tcagtggtgg tggtggtggt gctcgagcaa aaaacccctc aagacccgtt tagaggcccc
      181 aaggggttat gctagcttgc catgaatgat cccgaaggat catcagagta tgtgggagcc
      241 cacactctac tcgacagata cgaatatctg gacccgaccg tctcccacat acacatggca
      301 accctatagt gagtcgtatt aatttcgcgg gatcgagatc tcgatcctct acgccggacg
      361 catcgtggcc ggcatcaccg gcgccacagg tgcggttgct ggcgcctata tcgccgacat
      421 caccgatggg gaagatcggg ctcgccactt cgggctcatg agcgcttgtt tcggcgtggg
      481 tatggtggca ggccccgtgg ccgggggact gttgggcgcc atctccttgc atgcaccatt
      541 ccttgcggcg gcggtgctca acggcctcaa cctactactg ggctgcttcc taatgcagga
      601 gtcgcataag ggagagcgtc gagatcccgg acaccatcga atggcgcaaa acctttcgcg
      661 gtatggcatg atagcgcccg gaagagagtc aattcagggt ggtgaatgtg aaaccagtaa
      721 cgttatacga tgtcgcagag tatgccggtg tctcttatca g
//
