LOCUS       sequence-117291-        1079 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  N-terminal 400 amino acids of mouse BICD2 codon optimized for Sf9 
            expression with a N-terminal GFP tag and a TEV-cleavable His-ZZ tag 
            for purification (GFP-BICD2N).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1079)
  AUTHORS   Schlager MA, Hoang HT, Urnavicius L, Bullock SL, Carter AP
  TITLE     In vitro reconstitution of a highly processive recombinant human 
            dynein complex.
  JOURNAL   EMBO J. 2014 Sep 1;33(17):1855-68. doi: 10.15252/embj.201488792. 
            Epub 2014 Jul 1.
  PUBMED    24986880
REFERENCE   2  (bases 1 to 1079)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.15252/embj.201488792"; journalName: "EMBO J"; date: "2014-09-1- 
            1"; volume: "33"; issue: "17"; pages: "1855-68"
COMMENT     Sequence Label: GFP-BICD2N
FEATURES             Location/Qualifiers
     source          1..1079
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(59..92)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        169..260
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     185..202
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             327..350
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             393..566
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="VDNKFNKEQQNAYYEILHLPNLNEGQRNAFIQSLKDDPSQSANLL
                     AEAKKLNDAQAPK"
     CDS             567..740
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="VDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLL
                     AEAEKLNDAQAPK"
     CDS             798..818
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             840..860
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 cttcatccgt ttccacggtg tgcgtcaccc ggcaaccttg ggcagcagcg aagtcgccat
       61 aacttcgtat agcatacatt atacgaagtt atctgtaact ataacggtcc taaggtagcg
      121 agtttaaaca ctagtatcga ttcgcgacct actccggaat attaatagat catggagata
      181 attaaaatga taaccatctc gcaaataaat aagtatttta ctgttttcgt aacagttttg
      241 taataaaaaa acctataaat attccggatt attcataccg tcccaccatc gggcgcggat
      301 ctcggtccga aaccatgtcg tactaccatc accatcacca tcaccatcac ggttctatgt
      361 gcatggcagg ccttgcgcaa cacgatgaag ccgtggacaa caaattcaac aaagaacaac
      421 aaaacgcgta ctatgagatc ttacatttac ctaacttaaa cgaaggacaa cgaaacgcct
      481 tcatccaaag tttaaaagat gacccaagcc aaagcgctaa ccttttagca gaagctaaaa
      541 agctaaatga tgctcaggcg ccgaaagtag acaacaaatt caacaaagaa caacaaaacg
      601 cgttctatga gatcttacat ttacctaact taaacgaaga acaacgaaac gccttcatcc
      661 aaagtttaaa agatgaccca agccaaagcg ctaacctttt agcagaagct gaaaagctaa
      721 atgatgctca ggcgccgaaa gtagacgcaa attccgcggg gaagtcaacc gattatgata
      781 ttccaactac tgctagcgag aacctatatt tccagggaga ttatgatatc cctacgactg
      841 agaatcttta ttttcagggc atgtctaaag gtgaagaatt attcactggt gttgtcccaa
      901 ttttggttga attagatggt gatgttaatg gtcacaaatt ttctgtctcc ggtgaaggtg
      961 aaggtgatgc tacttacggt aaattgacct taaaatttat ttgtactact ggtaaattgc
     1021 cagttccatg gccaacctta gtcactactt tcggttatgg tgttcaatgt tttgcgaga
//
