LOCUS       sequence-117294-        1072 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  N-terminal 400 amino acids of mouse BICD2 codon optimized for Sf9 
            expression with a N-terminal GFP tag and a TEV-cleavable His-ZZ tag 
            for purification (GFP-BICD2N).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1072)
  AUTHORS   Schlager MA, Hoang HT, Urnavicius L, Bullock SL, Carter AP
  TITLE     In vitro reconstitution of a highly processive recombinant human 
            dynein complex.
  JOURNAL   EMBO J. 2014 Sep 1;33(17):1855-68. doi: 10.15252/embj.201488792. 
            Epub 2014 Jul 1.
  PUBMED    24986880
REFERENCE   2  (bases 1 to 1072)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.15252/embj.201488792"; journalName: "EMBO J"; date: "2014-09-1- 
            1"; volume: "33"; issue: "17"; pages: "1855-68"
COMMENT     Sequence Label: GFP-BICD2N
FEATURES             Location/Qualifiers
     source          1..1072
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(857..868)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 taagctgcaa taaacaagtt aacaacaaca attgcattca ttttatgttt caggttcagg
       61 gggaggtgtg ggaggttttt taaagcaagt aaaacctcta caaatgtggt atggctgatt
      121 atgatcctct agtacttctc gacaagctca ttacctcaga gcgctcagat tctcggtcaa
      181 cctgttcacc ttctcatgct gctctgacaa ggtacctcta gcctgctcca actgtttctg
      241 agtatcttgc aatgtggcca gcagacccac cttctcgcgc tccatttgga cgagctgctg
      301 cttgagcttc tggatttcgc tgatgtgcaa ctcgctcaac agatcggaca ccaaggaagg
      361 agagggagga gccagaccat ccttgcgtgg agtgctagtc ttgttatcca atgaggattt
      421 gaccaggccg ctatgttcga atccgttcac gagagcttcg gcatcattgt tgggctcggc
      481 ggtaacagtg tcgtcgctga acttcagtcc gtccaagctg acctggagat gtgaagtgta
      541 gaaggaatcg ttgatagaca tatagtggct caactccttg cggaggttat tcttctgctc
      601 cctttctgtc ttcagtgttt ccagggcttc ttcgagctgt ctttctgaaa tctccttcag
      661 tctgatagcg tcctccagtt gggagttcag gtactctgtc tcctcttcga ggcgcttgat
      721 ttcgtgcttc agtccttcga actccacctg attttgcctg agcactgaga cttgtttctg
      781 cagagagatg ttctcctctt ccagctctga gtagtcttgg aggagtcttg cctccctgaa
      841 tttgtattct ttgatgtcgt ccctcaatct accgcgttga atctcgacgt tctgattgat
      901 ttccttgagc tcttgggcca cgcttgtcaa tctctcgttt tcagattggg tgttggtcag
      961 aacgttcctg agttgcttca actcggtctg cagctccagg actttcctca cgtaatactg
     1021 ctccttgcta gcagactcct ggatcagtga ttcttctctg gactctccgt ct
//
