LOCUS       sequence-117295-         853 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Doxycycline-regulated mammalian expression vector for expressing 
            shRNA against E2F2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 853)
  AUTHORS   Hoog G, Zarrizi R, von Stedingk K, Jonsson K, Alvarado-Kristensson M
  TITLE     Nuclear localization of gamma-tubulin affects E2F transcriptional 
            activity and S-phase progression.
  JOURNAL   FASEB J. 2011 Nov;25(11):3815-27. doi: 10.1096/fj.11-187484. Epub 
            2011 Jul 25.
  PUBMED    21788450
REFERENCE   2  (bases 1 to 853)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1096/fj.11-187484"; journalName: "FASEB J"; date: "2011-11"; 
            volume: "25"; issue: "11"; pages: "3815-27"
COMMENT     Sequence Label: pTER E2F2shRNA human
FEATURES             Location/Qualifiers
     source          1..853
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    140..158
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     complement(204..223)
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     promoter        425..529
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(748..767)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 tcgagcggcc gccactgtgc tggatatctg cagaattcca ccacactgga ctagtggatc
       61 cgagctcggt accaagcttt tccaaaaaga ctcggtatga cacttcgtct cttgaacgaa
      121 gtgtcatacc gagtcgggat ctctatcact gatagggaac ttataagatt cccaaatcca
      181 aagacatttc acgtttatgg tgatttccca gaacacatag cgacatgcaa atattgcagg
      241 gcgccactcc cctgtccctc acagccatct tcctgccagg gcgcacgcgc gctgggtgtt
      301 cccgcctagt gacactgggc ccgcgattcc ttggagcggg ttgatgacgt cagcgttcga
      361 attcctgcag cccgggggat ctcccgatcc gtcgacgtca ggtggcactt ttcggggaaa
      421 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
      481 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
      541 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
      601 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
      661 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
      721 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
      781 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
      841 accagtcaca gaa
//
