LOCUS       sequence-117298-         866 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Puromycin-based homologous recombination-dependent reporter for 
            genome editing with TALENs or CRISPR/Cas9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 866)
  AUTHORS   Flemr M, Buhler M
  TITLE     Single-Step Generation of Conditional Knockout Mouse Embryonic Stem 
            Cells.
  JOURNAL   Cell Rep. 2015 Jul 28;12(4):709-16. doi: 
            10.1016/j.celrep.2015.06.051. Epub 2015 Jul 16.
  PUBMED    26190102
REFERENCE   2  (bases 1 to 866)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2015.06.051"; journalName: "Cell Rep"; date: 
            "2015-07-28- 28"; volume: "12"; issue: "4"; pages: "709-16"
COMMENT     Sequence Label: pMB1610_pRR-Puro
FEATURES             Location/Qualifiers
     source          1..866
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(8..604)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="TEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIERV
                     TELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLAA
                     QQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETSA
                     PRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(91..111)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     promoter        complement(838..856)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 cggccgctca ggcaccgggc ttgcgggtca tgcaccaggt gcgcggtcct tcgggcacct
       61 ctacgtcggc ggtgacggtg aagccgagcc gctcgtagaa ggggaggttg cggggcgcgg
      121 aggtctccag gaaggcgggc accccggcgc gctcggccgc ctccactccg gggagcacga
      181 cggcgctgcc cagacccttg ccctggtggt cgggcgagac gccgacggtg gccaggaacc
      241 acgcgggctc cttgggccgg tgcggcgcca ggaggccttc catctgttgc tgcgcggcca
      301 gccgggaacc gctcaactcg gccatgcgcg ggccgatctc ggcgaacacc gcccccgctt
      361 cgacgctctc cggcgtggtc cagaccgcca ccgcggcgcc gtcgtccgcg acccacacct
      421 tgccgatgtc gagcccgacg cgcgtgagga agagttcttg cagctcggtg acccgctcga
      481 tgtggcggtc cggatcgacg gtgtggcgcg tggcggggta gtcggcgaac gcggcggcga
      541 gggtgcgtac ggccctgggg acatcgtcgc gggtggcgag gcgcaccgtg ggcttgtact
      601 cggtttagac gtcaagagct ctcattacgc gacccacacc ttgccgatgt cgagcccgac
      661 gcgcgtgagg aagagttctt gcagctcggt gacccgctcg atgtggcggt ccggatcgac
      721 ggtgtggcgc gtggcggggt agtcggcgaa cgcggcggcg agggtgcgta cggccctggg
      781 gacatcgtcg cgggtggcga ggcgcaccgt gggcttgtac tcggtcatgg tggctagcct
      841 atagtgagtc gtattaagta ctctag
//
