LOCUS       sequence-117305-         850 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Doxycycline-regulated mammalian expression vector for expressing 
            shRNA against RB1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 850)
  AUTHORS   Ehlen A, Rossello CA, von Stedingk K, Hoog G, Nilsson E, Pettersson 
            HM, Jirstrom K, Alvarado-Kristensson M
  TITLE     Tumors with nonfunctional retinoblastoma protein are killed by 
            reduced gamma-tubulin levels.
  JOURNAL   J Biol Chem. 2012 May 18;287(21):17241-7. doi: 
            10.1074/jbc.M112.357038. Epub 2012 Apr 6.
  PUBMED    22493456
REFERENCE   2  (bases 1 to 850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M112.357038"; journalName: "J Biol Chem"; date: 
            "2012-05-18- 18"; volume: "287"; issue: "21"; pages: "17241-7"
COMMENT     Sequence Label: pTER RB1shRNA human
FEATURES             Location/Qualifiers
     source          1..850
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    137..155
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     complement(201..220)
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     promoter        422..526
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(745..764)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 gagcggccgc cactgtgctg gatatctgca gaattccacc acactggact agtggatccg
       61 agctcggtac caagcttttc caaaaagaac gattatccat tcaaatctct tgaatttgaa
      121 tgataatcgt tcgggatctc tatcactgat agggaactta taagattccc aaatccaaag
      181 acatttcacg tttatggtga tttcccagaa cacatagcga catgcaaata ttgcagggcg
      241 ccactcccct gtccctcaca gccatcttcc tgccagggcg cacgcgcgct gggtgttccc
      301 gcctagtgac actgggcccg cgattccttg gagcgggttg atgacgtcag cgttcgaatt
      361 cctgcagccc gggggatctc ccgatccgtc gacgtcaggt ggcacttttc ggggaaatgt
      421 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
      481 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
      541 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
      601 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
      661 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
      721 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
      781 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
      841 agtcacagaa
//
