LOCUS       sequence-117311-         715 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  co-expression of Cas9 and a sgRNA targeting 5'end of C.elegans 
            K08F4.2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 715)
  AUTHORS   Paix A, Wang Y, Smith HE, Lee CY, Calidas D, Lu T, Smith J, Schmidt 
            H, Krause MW, Seydoux G
  TITLE     Scalable and versatile genome editing using linear DNAs with 
            microhomology to Cas9 Sites in Caenorhabditis elegans.
  JOURNAL   Genetics. 2014 Dec;198(4):1347-56. doi: 10.1534/genetics.114.170423.
            Epub 2014 Sep 23.
  PUBMED    25249454
REFERENCE   2  (bases 1 to 715)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.114.170423"; journalName: "Genetics"; date: 
            "2014-12"; volume: "198"; issue: "4"; pages: "1347-56"
COMMENT     Sequence Label: AP332-3
FEATURES             Location/Qualifiers
     source          1..715
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(53..75)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_RNA        complement(347..422)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac
       61 acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag
      121 cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat
      181 cagagcagat tgcactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa
      241 ggagaaaata ccgcatcagg cgccattcgc cattcaggct gcgcaactgt tgggaagggc
      301 gatcggtgcg ggcctcttcg ctattacgcc agaaatttca caaaaagcac cgactcggtg
      361 ccactttttc aagttgataa cggactagcc ttattttaac ttgctatttc tagctctaaa
      421 acaggttatc tgcatttaat acaagacatc tcgcaatagg aggtgtttat ataggggcaa
      481 cacaagaggc ggcagacatc tcgtgagttt gacagagtag tataaagagt gtgtgtgaga
      541 gggtgtaggc atttcataac atattttttg tggaataatt ttggaaaaaa ggtgtttttt
      601 taataatagc ttttataaga gtttctcaaa aattgcatgc tttttttgaa aaggttgaca
      661 aattatagta gtactaaagt tgctattttc aggtctcagc agaaaattca caact
//
