LOCUS       sequence-117317-         349 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Parental plasmid for transcription of RNAs with a GlmS/MS2 3'-tag 
            for native purification.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 349)
  AUTHORS   Batey RT, Kieft JS
  TITLE     Improved native affinity purification of RNA.
  JOURNAL   RNA. 2007 Aug;13(8):1384-9. Epub 2007 Jun 4.
  PUBMED    17548432
REFERENCE   2  (bases 1 to 349)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "RNA."; 
            date: "2007-08"; volume: "13(8)"; pages: "1384-9. Epub 2007 Jun 4"
COMMENT     Sequence Label: pRAV23
FEATURES             Location/Qualifiers
     source          1..349
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     7..26
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        7..25
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      296..343
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 gaattctaat acgactcact atagggtcgc tcaaacatta agtggtgttg tgcgaaaaga
       61 atctcgaaga caagccggca tagcgcccga actaccggta ccggtagttg acgaggatgg
      121 aggttatcga attttcggcg gatgcctccc ggctgagtgt gcagatcaca gccgtaagga
      181 tttcttcaaa ccaagggggt gactccttga acaaagagaa atcacatgat cttccttttt
      241 tctagacgta caccatcagg gtacgttttt cagacaccat cagggtctgg gatccctagc
      301 ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgaagctt
//
